十字花科黑腐病菌中转录因子HpaR1与Clp调控一个糖苷水解酶基因表达的分析

刘国芳, 任沛东, 叶文新, 陆光涛

Analysis of transcriptional regulators HpaR1 and Clp regulating the expression of glycoside hydrolase-encoding gene in the Xanthomonas campestris pv. campestris

Liu Guofang, Ren Peidong, Ye Wenxin, Lu Guangtao

表1 本研究所用的菌株与质粒

Table 1 The strains and plasmids used in this study

菌株和质粒 相关特征 大肠杆菌 DH5α F-,φ80d/lacZ△M15,Δ(lacZYA-argF)U169,deoR,recA1,endA1,hsdR17(rk-, mk+),phoA,supE44, λ-,thi-1,gyrA96,relA1 M15 Nals,trs,Rifs,Thi-,Lac-,Ar+a,Gal+,Mtl-,F-,RecA+,Uvr+,Lon+harboring pREP4 plasmid,Kmr ED8767 RecA,met,含有帮助质粒 pRK2073,Spcr pQE-HpaR1/M15 M15中含有质粒pQE-HpaR1,Kmr,Ampr pQE-Clp/M15 M15中含有质粒 pQE-Clp,Kmr,Ampr pL3-HpaR1::3×Flg/ DH5α DH5α中含有质粒pL3-HpaR1:: 3×Flg,Tcr pL3-Clp:: 3×Flg/ DH5α DH5α中含有质粒pL3-Clp:: 3×Flg,Tcr 十字花科黑腐病菌 8004 野生型,Rifr ΔhpaR1 hpaR1基因的缺失突变体,Rifr Δclp Clp基因的缺失突变体,Rifr 8004/pLAFR3 8004中含有质粒pLAFR3,Rifr,Tcr ΔhpaR1/pL3-HpaR1::3×Flg ΔhpaR1中含有质粒 pL3-HpaR1::3×Flg,Rifr,Tcr Δclp/pL3-Clp:: 3×Flg Δclp中含有质粒 pL3-Clp:: 3×Flg,Rifr,Tcr 质粒 pK18mob 黄单胞菌属中的自杀质粒,Kmr pRK2073 帮助质粒,Tra+,Mob+,ColE1,Spcr pQE30 表达载体,Ampr pQE30-HpaR1 pQE30上含有hpaR1基因的360 bp ORF,Ampr pQE30-Clp pQE30上含有 clp基因的690 bp ORF,Ampr pLAFR3 PK2 replicon;Mob+,Tra-;cos+,Tcr pL3-HpaR1::3×Flg pLAFR3上含有 hpaR1::3×Flg,Tcr pL3-Clp::3×Flg pLAFR3上含有 clp::3×Flg,Tcr