十字花科黑腐病菌中转录因子HpaR1与Clp调控一个糖苷水解酶基因表达的分析

刘国芳, 任沛东, 叶文新, 陆光涛

Analysis of transcriptional regulators HpaR1 and Clp regulating the expression of glycoside hydrolase-encoding gene in the Xanthomonas campestris pv. campestris

Liu Guofang, Ren Peidong, Ye Wenxin, Lu Guangtao

表2 本研究所用的引物

Table 2 Primers used in this study

用途 引物名称 引物序列(5′→3′) 蛋白表达 Clp-OF ACAGTTGGATCC ATGAGCCTAGGGAACAGCAC Clp-OR ACAGTTAAGCTT TTAGCGCGTGCCGTACAACA 凝胶阻滞分析 0026 F ACGGCAATCGATCAGTTCGC 0026 R CATGGAAACCACTCCTTCGACG DNase I保护实验 0026(HpaR1)F GACAAGCCGCAGATGAAAACCC 0026(HpaR1)R GTTACGCTGCATCTGCCCGT 0026(Clp)F GCAGCCGGCGCACGCAGTAGACCTGTACT 0026(Clp)R CGACGGAAAAGATGCATCGCAGCCC 5ʹ-RACE AAP GGCCACGCGTCGACTAGTACGGGGGGGGGG AUAP GGCCACGCGTCGACTAGTAC 0026GSP1 GTTCCCACAGGATCGGCAGG 0026GSP2 TCCAGTCCCAGCGAAATAGC 0026GSP3 TGTTGAGGACGCCAGGTTTT 0026GSPF ATGTCGTGTTCTCTGTGGTTGC 体外转录 0026ivtF GCGACAAGCCGCAGATGAAAACCCT 0026ivtR GATGACGCAAATTCCGCACCCGCC 3077ivtF TCTCACTCTGTCTTGCAAACTGCGA 3077ivtR AATGGGCATCGAAAACCAGAAGC 染色质免疫共沉淀 Clp(ChIP)F CGGGATCCGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGACTACAA-AGATGACGACGATAAAATGAGCCTAGGGAACACGACG Clp(ChIP)R CCAAGCTTTTAGCGCGTGCCGTACAACA HpaR1(ChIP)F CGGGATCCGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGACTACAA-AGATGACGACGATAAAATGACTGACATCCAGTGGAGC HpaR1(ChIP)R CCAAGCTTTCATGGTGTTTTCCCCTGTG 0026(ChIP)F CGTCACGCTTTCGTCGCCTA 0026(ChIP)R AACTGCGTTGTGGCCTGCAC 实时荧光定量PCR 0026qRT-F ATGTCGTGTTCTCTGTGGTTGC 0026qRT-R TGTTGAGGACGCCAGGTTTT