Abstract: Total RNA was isolated from Chang Liver cell. The full-length cDNA was synthesized using LD PCR by RNA 5^，;end switching mechanism, oligo(dT) as primer. SfiI digestion sites were introduced at both 3^，;and 5^，;end of cDNA. The cDNA was ligated with λTriplEX2 vector and packaged. Twenty-two positive clones were obtained by immunoscreening to the library, they were sequenced and blast in NCBI, one of them was identified a novel gene. It was submitted to GenBank and obtained accession nubmer AY078070. It may lay down a foundation for studying on functions of ADAMs related gene.