普通小麦-纤毛鹅观草染色体异附加系的分子标记鉴定

doi:10.3724/SP.J.1005.2008.01356

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HEREDITAS ›› 2008, Vol. 30 ›› Issue (10): 1356-1362.doi: 10.3724/SP.J.1005.2008.01356

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Molecular marker analysis of wheat-Roegneria ciliaris additions lines

KONG Ling-Na; LI Qiao; WANG Hai-Yan; CAO Ai-Zhong; CHEN Pei-Du; WANG Xiu-E

National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China

Received:2008-01-15 Revised:2008-03-12 Online:2008-10-10 Published:2008-10-10
Contact: WANG Xiu-E xiu-e

Abstract

Abstract: A total of 135 EST, 27 STS, and 253 SSR primer pairs located in 7 homoeologous groups of wheat and barly were used for amplification of 24 possible Wheat-R. ciliaris disomic addition lines. Fifty-five primer pairs could amplified polymorphic bands between common wheat variety “Inayama Komugi” and the Inayama Komugi- R. ciliaris amphiploid, and 31 of the 55 could amplify the specific bands of R. ciliaris in the addition lines. According to the PCR (polymerase Chain Reaction) results, the added R. ciliaris chromosomes in lines 07K02, 07K06, 07K39, 07K201, 07K202, 07K255, and 07K256 belong to homoeologous group 1 of wheat; the added R. ciliaris chromosomes in lines 07K07, 07K08, 07K09, 07K11, 07K14, and 07K17 belong to homoeologous group 2 of wheat; the added R. ciliaris chromosomes in lines 07K15, 07K16, 07K21, and 07K47 belong to homoeologous group 6 of wheat

Key words: Triticum aestivum L., Roegneria ciliaris (Trin) Nevski, addition line, homoeologous group, EST, STS

KONG Ling-Na, LI Qiao, WANG Hai-Yan, CAO Ai-Zhong, CHEN Pei-Du, WANG Xiu-E. Molecular marker analysis of wheat-Roegneria ciliaris additions lines[J]. HEREDITAS, 2008, 30(10): 1356-1362.

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Molecular marker analysis of wheat-Roegneria ciliaris additions lines

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