Abstract: In this paper we studied changes of heterochromatin on the chromosomes of maize pollen haploid plants with Giemsa BSG C-banding technique.The main results abtai ned were as follows: The Giemsa C-banding of some haploid plants showed to be the same as that of donor.They had prominent heterochromatic bands on five of the ten chromosomes,w hich is half of the banding number of donor plants.Because the pollen haploid pl ants possessed only one set of chromosomes,the donor diploid plants possessed tw o sets of chromosomes.The other haploid plants had 3,4,6 and 7 bands,respectivel y.It is,therefore,considred that in invitro culture there are not only visible c hanges of chromosome number and structure,but also changes of chromosome heteroc hromatin.It showed that the number of heterochromatic chromacentres in nuclei at interphase stage was close to that of number of banding on chromosomes at metap hase stage.

Key words: Variation, Maize pollen haploid plant, Chromosome, Geterochromatin banding