CRISPR/Cas9系统在昆虫中的应用

doi:10.16288/j.yczz.17-263

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Hereditas(Beijing) ›› 2018, Vol. 40 ›› Issue (4): 266-278.doi: 10.16288/j.yczz.17-263

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Applications of the CRISPR/Cas9 system in insects

Xiaoling Tong^1,²,Chunyan Fang^1,²,Tingting Gai^1,²,Jin Shi^1,²,Cheng Lu^1,²,Fangyin Dai^1,²()

^1. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China
^2. Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture, College of Biotechnology, Southwest University, Chongqing 400715, China

Received:2017-10-16 Revised:2018-01-21 Online:2018-04-20 Published:2018-01-24
Contact: Dai Fangyin E-mail:fydai@swu.edu.cn
Supported by:
Supported by the The National High Technology Research and Development Program of China (863 Program)(2013AA102507);the National Natural Science Foundation of China(31472153);the National Natural Science Foundation of China(31372379);the Technical System Special of Modern Agricultural industry of China(CARS-18)

Abstract

Abstract:

The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system guides Cas9 to specific genomic locations by a short RNA search string. This technology enables the systematic interrogation of mammalian genome editing, repairing damaged genes, silencing harmful genes and improving quality traits. In recent years, with the introduction of the CRISPR/Cas9 system for easy, fast and efficient genetic modification, it has been possible to conduct meaningful functional studies in a broad array of insect species, such as Drosophila, Bombyx mori, Aedes aegypti and butterflies et al. In this review, we summarize the application of CRISPR/Cas9 in different insect species, discuss methods for its promotion, and consider its application for future insect studies.

Key words: CRISPR/Cas9, genome editing, insects, application

Xiaoling Tong,Chunyan Fang,Tingting Gai,Jin Shi,Cheng Lu,Fangyin Dai. Applications of the CRISPR/Cas9 system in insects[J]. Hereditas(Beijing), 2018, 40(4): 266-278.

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