低温变性下复合PCR技术及其应用

doi:10.16288/j.yczz.17-369

[an error occurred while processing this directive]

Hereditas(Beijing) ›› 2018, Vol. 40 ›› Issue (3): 227-236.doi: 10.16288/j.yczz.17-369

• Reviews • Previous Articles Next Articles

Co-amplification at lower denaturation temperature-PCR: methodology and applications

Hui Liang^1,²,Guojie Chen³,Yan Yu⁴,Likuan Xiong^1,²()

^1. Central Laboratory, Bao’an Maternal and Child Health Hospital, Jinan University, Shenzhen 518102, China;
^2. Shenzhen Key Laboratory of Birth Defects, Shenzhen 518102, China
^3. Department of Gastroenterology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
^4. Department of Obstetrics, Bao’an Maternal and Child Health Hospital, Jinan University, Shenzhen 518102, China;

Received:2017-11-06 Revised:2018-02-02 Online:2018-03-20 Published:2018-02-08
Supported by:
[Supported by Sanming Project of Medicine in Shenzhen-Brith Defects Prevention Research and Transformation Team (No. SZSM201406007), Shenzhen Key Laboratory of Birth Defects (No. ZDSYS201504301707152 and Science and Technology Plan Project of Baoan District (Nos. 2014067, 2017JD001)]

Abstract

Abstract:

Co-amplification at lower denaturation temperature-polymerase chain reaction (COLD-PCR) is a novel form of PCR that selectively denatures and amplifies low-abundance mutations from mixtures of wild-type and mutation-containing sequences, enriching the mutation 10 to 100 folds. Due to the slightly altered melting temperature (Tm) of the double-stranded DNA and the formation of the mutation/wild-type heteroduplex DNA, COLD-PCR methods are sensitive, specific, accurate, cost-effective and easy to maneuver, and can enrich mutations of any type and at any position, even unknown mutations within amplicons. COLD-PCR and its improved methods are now applied in cancer, microorganisms, prenatal screening, animals and plants. They are extremely useful for early diagnosis, monitoring the prognosis of disease and the efficiency of the treatment, drug selection, prediction of prognosis, plant breeding and etc. In this review, we introduce the principles, key techniques, derived methods and applications of COLD-PCR.

Key words: co-amplification at lower denaturation temperature polymerase chain reaction (COLD-PCR), Tc, selectively amplifies, low-abundance mutation, detection threshold

Hui Liang, Guojie Chen, Yan Yu, Likuan Xiong. Co-amplification at lower denaturation temperature-PCR: methodology and applications[J]. Hereditas(Beijing), 2018, 40(3): 227-236.

References

[1]	Lu YW, Wang KH . Research progress on genetic heterogeneity between primary and paired metastatic colorectal cancer. Hereditas (Beijing), 2017,39(6):482-490.
[1]	鲁有望, 王昆华 . 结直肠癌原发与配对转移肿瘤遗传异质性研究进展. 遗传, 2017,39(6):482-490.
[2]	Milbury CA, Li J, Makrigiorgos GM . PCR-based methods for the enrichment of minority alleles and mutations. Clin Chem, 2009,55(4):632-640.
[3]	Li J, Wang LL, Mamon H, Kulke MH, Berbeco R, Makrigiorgos GM . Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing. Nat Med, 2008,14(5):579-584.
[4]	How Kit A, Mazaleyrat N, Daunay A, Nielsen HM, Terris B, Tost J . Sensitive detection of KRAS mutations using enhanced-ice-COLD-PCR mutation enrichment and direct sequence identification. Hum Mutat, 2013,34(11):1568-1580.
[5]	Castellanos-Rizaldos E, Richardson K, Lin R, Wu G, Makrigiorgos MG . Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR. Clin Chem, 2015,61(1):267-277.
[6]	Yuan F, Xu J, Ji LD, Fei LJ, Liu PP, Zhang LN . Application of Tm-shift genotyping method in genetic studies. Hereditas (Beijing), 2012,34(11):1484-1490.
[6]	袁芳, 徐进, 季林丹, 费丽娟, 刘盼盼, 张莉娜 . Tm- shift基因分型方法在遗传学中的应用. 遗传, 2012,34(11):1484-1490.
[7]	Castellanos-Rizaldos E, Paweletz C, Song C, Oxnard GR, Mamon H, J?nne PA, Makrigiorgos GM . Enhanced ratio of signals enables digital mutation scanning for rare allele detection. J Mol Diagn, 2015,17(3):284-292.
[8]	Milbury CA, Correll M, Quackenbush J, Rubio R, Makrigiorgos GM . COLD-PCR enrichment of rare cancer mutations prior to targeted amplicon resequencing. Clin Chem, 2012,58(3):580-589.
[9]	Li J, Milbury CA, Li C, Makrigiorgos GM . Two- round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma. Hum Mutat, 2009,30(11):1583-1590.
[10]	Kristensen LS, Daugaard IL, Christensen M, Hamilton-Dutoit S, Hager H, Hansen LL . Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products. Hum Mutat, 2010,31(12):1366-1373.
[11]	Hashida S, Soh J, Toyooka S, Tanaka T, Furukawa M, Shien K, Yamamoto H, Asano H, Tsukuda K, Hagiwara K, Miyoshi S . Presence of the minor EGFR T790M mutation is associated with drug-sensitive EGFR mutations in lung adenocarcinoma patients. Oncol Rep, 2014,32(1):145-152.
[12]	Milbury CA, Li J, Makrigiorgos GM . Ice-COLD-PCR enables rapid amplification and robust enrichment for low- abundance unknown DNA mutations. Nucleic Acids Res

Metrics

Comments

www.chinagene.cn
备案号：京ICP备09063187号

Co-amplification at lower denaturation temperature-PCR: methodology and applications

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments