Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China
Received date: 2007-01-29
Revised date: 2007-04-05
Online published: 2007-10-10
In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers (including SSR and STS primers) were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the analyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the region of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow (from short to long): IGV1-465
JI Jian-Hui, CAO Ai-Zhong, WANG Hai-Yan, QIN Bi, WANG Su-Ling, KONG Fang, CHEN Pei-Du, LIU Da-Jun, WANG Xiu-E . Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers[J]. Hereditas(Beijing), 2007 , 29(10) : 1256 -1256―1262 . DOI: 10.1360/yc-007-1256
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