With the proteomics concept introduction and effective projects implement as Human Plasma Project, the proteomics research is being developed rapidly, which is based on the analytical chemistry and physical chemistry. More and more biologists paid attention to this field, and got some important results in cell biology and biochemistry. The variation of protein quantity and various modifications in the cell reflects the environment stress and the function requirement. To inves-tigate these variations, proteomic technology provided powerful tools. In this article, chemical probe in quantitative and modification study is discussed in details.