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研究报告

Cloning and analyzing of rice blast resistance gene Pi-ta allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan

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  • 1. School of Life Science, Yunnan University, Kunming 650091, China;
    2. Biotechnology and Germplasm Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;
    3. Agricultural Institute of Xishuangbanna Prefecture, Jinghong, Yunnan Province 666100, China

Received date: 2007-06-08

  Revised date: 2007-08-20

  Online published: 2008-01-10

Abstract

A 4 672 bp DNA sequence including the whole coding region and partial non-coding region of rice blast
resistance gene Pi-ta+ has been cloned from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan by polymerase chain reaction method. The coding region shares 99.86% and 98.78% identity with the corresponding regions of the reported cultivated rice Yashiro-mochi and Yuanjiang type of common wild rice respectively. There are 4 nucleotides difference in the coding region and 6 in intron of the cloned Pi-ta+ gene,compared with Pi-ta from Yashiro-mochi. Pi-ta+ gene in Jinghong erect type of common wild rice has been proved to be a rare existing Pi-ta+ allele, because there was a alanine rather than a serine at the position 918 within the predicted amino acid sequence of PITA. Pi-ta+ allele can cause disease resistance response to rice blast pathogens in plant cells. Differences in DNA sequence, deduced amino acid se-quence and antibacterial spectrum may make the Pi-ta+ allele new resistant characteristics. Finding and cloning of Pi-ta+ allele from Jinghong erect type of common wild rice in Yunnan provides a basement for further utilization of the wild rice resources.

Cite this article

GENG Xian-Sheng, YANG Ming-Zhi, HUANG Xing-Qi, CHENG Zai-Quan, FU Jian, SUN Tao, LI Jun . Cloning and analyzing of rice blast resistance gene Pi-ta allele from Jinghong erect type of common wild rice (Oryza rufipogon Griff) in Yunnan[J]. Hereditas(Beijing), 2008 , 30(1) : 109 -114 . DOI: 10.3724/SP.J.1005.2008.00109

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