Improvement of the silver-stained technique of polyacrylamide gel electrophoresis

LIANG Hong-Wei, WANG Chang-Zhong, LI Zhong, : LUO Xiang-Zhong, ZOU Gui-Wei

doi:10.3724/SP.J.1005.2008.01379

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Hereditas(Beijing) >

2008 , Vol. 30 >Issue 10: 1379 - 1382

DOI: https://doi.org/10.3724/SP.J.1005.2008.01379

技术与方法

Improvement of the silver-stained technique of polyacrylamide gel electrophoresis

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1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China;
2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
3. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2008-03-28

Revised date: 2008-08-01

Online published: 2008-10-10

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Abstract

Abstract: Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.

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LIANG Hong-Wei, WANG Chang-Zhong, LI Zhong, : LUO Xiang-Zhong, ZOU Gui-Wei . Improvement of the silver-stained technique of polyacrylamide gel electrophoresis[J]. Hereditas(Beijing), 2008 , 30(10) : 1379 -1382 . DOI: 10.3724/SP.J.1005.2008.01379

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