Molecular cloning and mutation site analysis of <EM>AMPD1</EM> gene in swine

LIU Hui, XU Yao, ZHAO Li-Li, YANG Xiu-Qin, LIU Di

doi:10.3724/SP.J.1005.2008.00175

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Hereditas(Beijing) >

2008 , Vol. 30 >Issue 2: 175 - 178

DOI: https://doi.org/10.3724/SP.J.1005.2008.00175

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Molecular cloning and mutation site analysis of AMPD1 gene in swine

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1. College of Animal Science Technology, Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Received date: 2007-08-19

Revised date: 2007-11-28

Online published: 2008-02-10

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Abstract

Adenosine monophosphate deaminase gene (AMPD1) plays an important role in the purine nucleotide cycle.The AMPD1 gene is expressed predominantly in skeletal muscles. It related with Inosine monophosphate metabolism in muscles and carcass traits. Herein the AMPD1 gene was cloned and a CDS sequence of 2 195 bp was obtained and analyzed with PCR-SSCP. χ2 analysis showed that the distribution of genotypes among Min pig, Yorkshire and Duroc are extremely sig-nificant different (P<0.01).

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LIU Hui, XU Yao, ZHAO Li-Li, YANG Xiu-Qin, LIU Di . Molecular cloning and mutation site analysis of AMPD1 gene in swine[J]. Hereditas(Beijing), 2008 , 30(2) : 175 -178 . DOI: 10.3724/SP.J.1005.2008.00175

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