The computer-assisted software for population genetics’ teaching and research is designed with Microsoft Visual Basic 6.0. It takes all factors affecting population genetic structure into account. By way of setting various conditional parameters, calculating theoretically, simulating with computer and choosing the states of show, the system displays the results in charts and tables accurately. It also presents distribution pattern, statistical characteristics and the change of population gene frequencies and genotype frequencies in the inter-generation visually. The software is a mature system for teaching and researches on the aspects of its functions, operations and interface.