Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus

REN Hong-Lin, ZHANG Wen-Hui, PAN Feng-Guang, LU Shi-Ying, LIU Zeng-Shan, ZHANG Jun-Hui

doi:10.3724/SP.J.1005.2009.00180

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Hereditas(Beijing) >

2009 , Vol. 31 >Issue 2: 180 - 185

DOI: https://doi.org/10.3724/SP.J.1005.2009.00180

研究报告

Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus

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1. Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, China;
2. College of Light Industry and Economic, Jilin University, Changchun 130062, China;
3. Fujian Institute of Subtropical Botany, Xiamen 361006, China

Received date: 2008-05-06

Revised date: 2008-09-04

Online published: 2009-02-10

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Abstract

In this paper, we successfully constructed the cDNA library of major ampullate gland of Araneus ventricousus using pUC18 vector and cloned the partial cDNA (AvMaSp1, GenBank accession number AY177203) encoding spider ma-jor ampullate gland spidroin-1 by means of picking colony randomly (Bird gun). The partial cDNA sequence of AvMaSp1 was 1 408 bp and its region in encoding was 1 288 bp. The protein deduced from AvMaSp1 contained 429 amino acid resi-dues and molecular weight was 34.07 kDa. The repetitive motif of this cDNA sequence was (GA)nAm(GA)N and the identity with A. diadematus fibroin-1 mRNA (ADF-1, GenBank accession number ADU47853) was 75.0%.

Cite this article

REN Hong-Lin, ZHANG Wen-Hui, PAN Feng-Guang, LU Shi-Ying, LIU Zeng-Shan, ZHANG Jun-Hui . Cloning and sequence analysis of major ampullate spidroin-1 partial cDNA from Araneus ventricosus[J]. Hereditas(Beijing), 2009 , 31(2) : 180 -185 . DOI: 10.3724/SP.J.1005.2009.00180

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