Biochemical methods for the analysis of DNA-protein interactions

ZHANG Jin-Bi, PAN Zeng-Xiang, LIN Fei, MA Xue-Shan, LIU Hong-Lin

doi:10.3724/SP.J.1005.2009.00325

[an error occurred while processing this directive]

Hereditas(Beijing) >

2009 , Vol. 31 >Issue 3: 325 - 366

DOI: https://doi.org/10.3724/SP.J.1005.2009.00325

技术与方法

Biochemical methods for the analysis of DNA-protein interactions

Expand

College of animal science and technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2008-07-18

Revised date: 2008-12-08

Online published: 2009-03-10

Fold

Abstract

Investigation of DNA-protein interactions is fundamental to understand the mechanism underlying a variety of life processes. In this article, various types of biochemical methods in DNA-protein interaction study in vivo and in vitro at the level of DNA, protein, and the complex, respectively were briefly reviewed. Traditional assays including Nitrocellulose filter-binding assay, Footprinting, EMSA, and Southwestern blotting were summarized. In addition, chromatin immunopre-cipitation techniques including nChIP, xChIP, and ChIP-on-chip, which were widely used in epigenetics, were particularly introduced.

Cite this article

ZHANG Jin-Bi, PAN Zeng-Xiang, LIN Fei, MA Xue-Shan, LIU Hong-Lin . Biochemical methods for the analysis of DNA-protein interactions[J]. Hereditas(Beijing), 2009 , 31(3) : 325 -366 . DOI: 10.3724/SP.J.1005.2009.00325

Options

Outlines

〈

〉

www.chinagene.cn
备案号：京ICP备09063187号

模态框（Modal）标题

Abstract

Cite this article