Single nucleotide polymorphism (SNP), which is one of the common variations in the human genome, plays important roles in disease susceptibility and drug metabolism. Several genotyping methods have been established and ap-plied in SNP study. Single base extension (SBE) is one of SNP genotyping technologies. In this study, we reported a SNP detection method, which combined SBE and Zip-code microarray technologies. The results will provide a tool for develop-ing products that can be used for personal medicine and clinical diagnosis.