Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis

GAO Dong, DU Fei, ZHU You-Yong

doi:10.3724/SP.J.1005.2009.00668

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Hereditas(Beijing) >

2009 , Vol. 31 >Issue 6: 668 - 673

DOI: https://doi.org/10.3724/SP.J.1005.2009.00668

技术与方法

Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis

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The National Center for Agricultural Biodiversity, Ministry of Education Key Laboratory of Agricultural Biodiversity for Plant Disease Management, Key laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650201, China

Received date: 2008-11-04

Revised date: 2008-11-28

Online published: 2009-06-10

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Abstract

The disadvantage of time-consuming and fussy steps of conventional silver-stained method for polyacrylamide gels is evident, and has become the choke point in its application. In this paper, a low-background and high-resolution con-tracted silver-stained method for polyacrylamide gels was established. Compared with the conventional banding method, this contracted method has fewer steps and needs fewer reagents, in particular, lower NaOH concentration. It is rapid and economic, especially for mass silver-staining.

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GAO Dong, DU Fei, ZHU You-Yong . Low-background and high-resolution contracted silver-stained method in polyacrylamide gels electrophoresis[J]. Hereditas(Beijing), 2009 , 31(6) : 668 -673 . DOI: 10.3724/SP.J.1005.2009.00668

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