Molecular identification on Waxy genes in wheat using multiple-PCR

LU Long-Dou, HOU Cai-Ling, CHEN Long, YIN Gui-Hong, DENG Chuan-Liang, GAO Wu-Jun, YANG Xu-Qin, TAN Guang-Xuan

doi:10.3724/SP.J.1005.2009.00844

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Hereditas(Beijing) >

2009 , Vol. 31 >Issue 8: 844 - 848

DOI: https://doi.org/10.3724/SP.J.1005.2009.00844

研究报告

Molecular identification on Waxy genes in wheat using multiple-PCR

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1. College of Life Science, Henan Normal University, Xinxiang 453007, China;
2. Department of Life Science, Zhoukou Normal University, Henan Province Zhoukou 466000, China;
3. Zhoukou Institute of Agricultural Science, Henan Province Zhoukou 466001, China

Received date: 2008-12-23

Revised date: 2009-04-05

Online published: 2009-08-10

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Abstract

Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected si-multaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality proper-ties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.

Cite this article

LU Long-Dou, HOU Cai-Ling, CHEN Long, YIN Gui-Hong, DENG Chuan-Liang, GAO Wu-Jun, YANG Xu-Qin, TAN Guang-Xuan . Molecular identification on Waxy genes in wheat using multiple-PCR[J]. Hereditas(Beijing), 2009 , 31(8) : 844 -848 . DOI: 10.3724/SP.J.1005.2009.00844

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