Separation of the up-regulated genes under nitrogen starvation from <EM>Phaeodactylum tricornutum </EM>by suppression subtractive hybridization technology

TANG Jian-Xin, CHEN Zhuo, HU Han-Hua

doi:10.3724/SP.J.1005.2009.00865

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Hereditas(Beijing) >

2009 , Vol. 31 >Issue 8: 865 - 870

DOI: https://doi.org/10.3724/SP.J.1005.2009.00865

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Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology

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1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

Received date: 2009-02-13

Revised date: 2009-05-25

Online published: 2009-08-10

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Abstract

Sixteen cDNA fragments of Phaeodactylum tricornutum were isolated and identified to be up-regulated under nitrogen starvation by suppression subtractive hybridization (SSH) technology in order to elucidate the molecular mecha-nism of the diatom nitrogen utilization. Seven of them have high similarity with the functional genes related to the utiliza-tion of nitrogen. Northern blotting analysis indicated that 5 genes, nitrate transporter gene (nrt), nitrite reductase gene (nir), ammonium transporter gene (amt), ATP-binding cassette transporter gene (abc), and purine permease gene (pup), were significantly up-regulated under nitrogen starvation.

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TANG Jian-Xin, CHEN Zhuo, HU Han-Hua . Separation of the up-regulated genes under nitrogen starvation from Phaeodactylum tricornutum by suppression subtractive hybridization technology[J]. Hereditas(Beijing), 2009 , 31(8) : 865 -870 . DOI: 10.3724/SP.J.1005.2009.00865

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