The polymorphism of bovine POMC gene and its association with the growth traits of Nanyang cattle
Received date: 2009-08-16
Revised date: 2009-09-30
Online published: 2009-12-10
Pro-opiomelanocortin (POMC) plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds (i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein), and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle. Three SNPs (811845 C>T, 811821 T>C and 811797 A>G, ref. NW_928357) in linkage were detected in P3 locus, which were located at the 3’ flanking region of bovine POMC gene. The body weight at 6-month-old and the average daily gain of 0~6-month-old were higher in Nanyang cattle with the genotype BB than with the genotype AA (P<0.05).
Key words： cattle; POMC gene; SNP; growth trait; body weight
ZHANG Chun-Lei, WANG Yan-Gong, CHEN Hong, LEI Chu-Chao, FANG Xin-Tang, WANG Ji-Jiang, MA Gui-Bian, NIU Hui, XIAO Jie . The polymorphism of bovine POMC gene and its association with the growth traits of Nanyang cattle[J]. Hereditas(Beijing), 2009 , 31(12) : 1221 -1225 . DOI: 10.3724/SP.J.1005.2009.01.002
[1] Cai G, Cole SA, Bastarrachea RA, Maccluer JW, Blangero J, Comuzzie AG. Quantitative trait locus determining dietary macronutrient intakes is located on human chromosome 2p22. Am J Clin Nutr, 2004, 80(5): 1410–1414.
[2] Rotimi CN, Comuzzie AG, Lowe WL, Luke A, Blangero J, Cooper RS. The quantitative trait locus on chromosome 2 for serum leptin levels is confirmed in African-Americans. Dia-betes, 1999, 48(3): 643–644.
[3] Hager J, Dina C, Francke S, Dubois S, Houari M, Vatin V, Vaillant E, Lorentz N, Basdevant A, Clement K, Guy-Grand B, Froguel P. A genome-wide scan for human obesity genes reveals a major susceptibility locus on chromosome 10. Nat Genet, 1998, 20(3): 304–308.
[4] Montangue CT, Farooqi IS, Whitehead JP, Soos MA, Rau H, Wareham NJ, Sewter CP, Digby JE, Mohemmaed SN, Hurst JA, Cheetham CH, Earley AR, Barnett AH, Prins JB, O’Rahilly S. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature, 1997, 387(6636): 903–908.
[5] Clément K, Vaisse C, Lahlou N, Cabrol S, Pelloux V, Cassuto D, Gourmelen M, Dina C, Chambaz J, Lacorte JM, Basdevant A, Bougnères P, Lebouc Y, Froguel P, Guy-Grand B. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature, 1998, 392 (6674): 398–401.
[6] Thue TD, Buchanan FC. Linkage mapping of POMC to bo-vine chromosome 11. Anim Genet, 2003, 34(2): 146–160.
[7] Buchanan FC, Thue TD, Yu P, Winkelman-Sim DC. Single nucleotide polymorphisms in the corticotrophin-releasing hormone and pro-opiomelancortin genes are associated with growth and carcass yield in beef cattle. Anim Genet, 2005, 36(2): 127–131.
[8] Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX. POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre University of Alberta Canada, 1997.
[9] Hixson JE, Almasy L, Cole S, Birnbaum S, Mitchell BD, Mahaney MC, Stern MP, MacCluer JW, Blangero J, Comuz-zie AG. Normal variation in leptin levels is associated with polymorphisms in the proopiomelanocortin gene, POMC. J Clin End Metab, 1999, 84(9): 3187–3191. [10] De Vos P, Saladin R, Auwerx J, Staels B. Induction of ob gene expression by corticosteroids is accompanied by body weight loss and reduced food intake. J Biol Chem, 1995, 270(27): 15958–15961.
[11] Murakami T, Iida M, Shima K. Dexamethasone regulates obese expression in isolated rat adipocytes. Biochem Bio-phys Res Commun, 1995, 214(3): 1260–1267.
[12] Baker M, Gaukrodger N, Mayosi BM, Imrie H, Farrall M, Wat-kins H, Connell JM, Avery PJ, Keavney B. Association between common polymorphisms of the proopiomelanocortin gene and body fat distribution. Diabetes, 2005, 54(8): 2492–2496.
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号
