Fine mapping of mutant gene related corneal opacity mouse with SNPs
Received date: 2009-09-29
Revised date: 2009-12-11
Online published: 2010-05-15
To further investigate the genetic mechanism of the mutant mice(B6-Co) with hereditary corneal opacity phenotype obtained by ENU-induced mutagenesis from B6 in previous study, SNP markers were used to map the mutant gene of B6-Co mice. F2 generation mice were bred by backcrossing (B6-CoP×D2 )F1 with D2 and the DNA samples of F2 mutant mice were extracted from the tails. Five SNP sites that showed differences between B6 and D2 strains nearby the located region on chromosome 13 were screened from MGI database. Five SNPs, PCR-RFLP and linkage analyses were carried out to map the mutant gene. The result showed that the mutant gene was located between 112 546 283~113 397 654 bp on chromosome 13. There are five identified genes including Map3k1 that is associated with eye morphogenesis and eyelid closure of mouse in this region. This suggests that Map3k1 is the most probable candidate mutant gene of B6-Co mice.
Key words： SNP; corneal opacity mice; mutant gene; gene mapping
JIANG Ying-Mei, LIU Chun, TUN Liu-Cheng, SHAO Xi-Xiang . Fine mapping of mutant gene related corneal opacity mouse with SNPs[J]. Hereditas(Beijing), 2010 , 32(5) : 486 -491 . DOI: 10.3724/SP.J.1005.2010.00486
[1] 邵义祥, 吴宝金, 薛整风, 陈兵, 茅慧华, 赵明, 李厚达. 遗传性角膜基质变性小鼠及其突变基因的定位. 南京师大学报(自然科学版), 2006, 29(2): 99-102.
[2] 殷小敏. B6-Co小鼠角膜病变的病因和病理学研究[学位论文]. 南通大学, 2007.
[3] 王新蕾. 角膜混浊小鼠突变基因的进一步定位及其免疫学功能的初步研究[学位论文]. 南通大学, 2008.
[4] Sambrook J, Russell DW. 分子克隆实验指南. 第三版. 北京: 科学出版社, 2002, 479-483.
[5] Horikawa Y, Oda N, Cox NJ, Li X, Orho-Melander M, Hara M, Hinokio Y, Lindner TH, Mashima H, Schwarz PE, del Bosque-Plata L, Horikawa Y, Oda Y, Yoshiuchi I, Colilla S, Polonsky KS, Wei S, Concannon P, Iwasaki N, Schulze J, Baier LJ, Bogardus C, Groop L, Boerwinkle E, Hanis CL, Bell GI. Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus. Nat Genet, 2000, 26(2): 163-175.
[6] 关洪英, 唐志权, 李辉. 鸡MD基因5′侧翼区多态性与鸡生长和体组成性状的相关研究. 遗传学报, 2006, 33(6): 501-506.
[7] 翟光华, 闻平, 郭兰芳, 陈璐. Ⅱ型糖尿病患者APOA5-1131T/C基因多态性与血脂代谢和胰岛素抵抗的关系研究. 遗传, 2007, 29(5): 541-546.
[8] 郭晓令, 陈哲, 赵晓枫, 徐宁迎. SIM1基因第8外显子的SNP对猪背膘厚的影响. 遗传, 2008, 30(6): 7557-7559.
[9] 徐倩, 孙丽萍, 宫月华, 徐莹, 董楠楠, 袁媛. 粘蛋白MUC1 568A/G SNP与辽宁地区人群胃癌遗传易感性的关系. 遗传, 2008, 30(9): 1163-1168.
[10] Gerits N, Kostenko S, Moens U. In vivo functions of mi-togen-activated protein kinases: conclusions from knock-in and knock-out mice. Transgenic Res, 2007, 16(3): 281-314.
[11] Zhang L, Wang W, Hayashi Y, Jester JV, Birk DE, Gao M, Liu CY, Kao WW, Karin M. A role for MEK kinase 1 in TGF-β/activin-induced epithelium movement and embryonic eyelid closure. EMBO J, 2003, 22(17): 4443-4454.
[12] Martin P, Parkhurst SM. Parallels between tissue repair and embryo morphogenesis. Development, 2004, 131(13): 3021-3034.
[13] Gates A. lg
[14] Juriloff DM, Harris MJ, Mah DG. The open-eyelid muta-tion, lidgap-Gates, is an eight-exon deletion in the mouse Map3k1 gene. Genomics, 2005, 85(1): 139-142.
[15] Yujiri T, Ware M, Widmann C, Oyer R, Russell D, Chan E, Zaitsu Y, Clarke P, Tyler K, Oka Y, Fanger GR, Henson P, Johnson GL. MEK kinase 1 gene disruption alters cell migration and c-Jun NH2-terminal kinase regulation but does not cause a measurable defect in NF-kappa B activa-tion. Proc Natl Acad Sci USA, 2000, 97(13): 7272-7277.
[16] Xia Y, Makris C, Su B, Li E, Yang J, Nemerow GR, Karin M. MEK kinase 1 is critically required for c-Jun N-terminal kinase activation by proinflammatory stimuli and growth factor-induced cell migration. Proc Natl Acad Sci USA, 2000, 97(10): 5243-5248.
[17] Weston CR, Wong A, Hall JP, Goad ME, Flavell RA, Davis RJ. The c-Jun NH2-terminal kinase is essential for epidermal growth factor expression during epidermal morphogenesis. Proc Natl Acad Sci USA, 2004, 101(39): 14114-14119.
[18] Li G, Gustafson-Brown C, Hanks SK, Nason K, Arbeit JM, Pogliano K, Wisdom RM, Johnson RS. c-Jun is essential for organization of the epidermal leading edge. Dev Cell , 2003, 4(6): 865-877.
[19] Carroll JM, Luetteke NC, Lee DC, Watt FM. Role of in-tegrins in mouse eyelid development: studies in normal embryos and embryos in which there is a failure of eyelid fusion. Mech Dev, 1998, 78(1-2): 37-45.
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号
