[an error occurred while processing this directive]
en

Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

Expand
  • 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China; 2. Rubber Cultivation Research Institute, Chinese Academy of Tropic Agricultural Science, Danzhou 571737, China; 3. Qiongzhou University, Wuzhishan 572200, China; 4. Agricultural College, Hainan University, Haikou 570228, China

Received date: 2009-10-16

  Revised date: 2010-01-05

  Online published: 2010-08-23

Abstract

Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers, 176 were used for constructing the genetic map of rubber tree (Hevea brasiliensis) by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873. Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1:1 and 12 loci followed a ratio of 1:2:1 and 17 loci followed a ratio of 1:1:1:1. Only 13 (7.38%) loci were distorted from the Mendelian ratio. The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers. The largest linkage group consisted of 16 marker loci, while the smallest one contained only 2 marker loci.

Cite this article

FENG Su-Ping, LI Wei-Guo, XU Fei, WANG Jing-Yi, WU Yao-Ting . Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers[J]. Hereditas(Beijing), 2010 , 32(8) : 857 -863 . DOI: 10.3724/SP.J.1005.2010.00857

References

[1] Paglia GP, Olivieri AM, Morgante M. Towards sec-ond-generation STS (sequence-tagged sites) linkage maps in conifers: a genetic map of Norway spruce (Picea abies K.). Mol Gen Genet, 1998, 258(5): 466–478. [2] 安泽伟, 赵彥宏, 程汉, 李维国, 黄华孙. 橡胶树EST-SSR标记的开发和应用. 遗传, 2009, 31(3): 311-319. [3] Brown GR, Kadel III EE, Bassoni DL, Kiehne KL, Te-mesgen B, van Buijtenen JP, Swell MM, Marshall KA, Neal DB. Anchored reference loci in loblolly pine (Inus teada L.) for integrating pine genomics. Genetics, 2001, 159(2): 799–809. [4] Lespinasse D, Rodier-Goud M, Grivet L, Leconte A, Leg-nate H, Seguin M. A saturated genetic linkage map of rubber tree (Hevea spp.) based on RFLP, AFLP, microsa-tellite, and isozyme markers. Theor Appl Genet, 2000, 100: 127–138. [5] Venkatachalam P, Priya , Amma CK, Thulaseedharan A. Identification, cloning and sequencing analysis of a dwarf genomic-specific RAPD marker in rubber tree [Hevea brasiliensis (Muell.) Arg.]. Plant Cell Rep, 2004, 23(5): 327–332. [6] 陈守才, 邵寒霜, 胡东琼. 用RAPD技术鉴定橡胶树抗白粉病基因连锁标记. 热带作物学报, 1994, 15(2): 21–26. [7] Le Guen V, Lespinasse D, Oliver G, Rodier-Groud M, Pi-nard F, Seguin M. Molecular mapping of genes conferring field resistance to South American Leaf Blight (Microcyclus ulei) in rubber tree. Theor Appl Genet, 2003, 108(1): 160–167. [8] 和丽岗. 橡胶树分子遗传图谱的构建及相关性状的QTL初步定位[学位论文]. 华南热带农业大学, 2007. [9] 王惠君. 橡胶树初级分子遗传图谱的构建[学位论文]. 华南热带农业大学, 2007. [10] Venkatachalam P, Thomas S, Priya P, Thanseem I, Gireesh T,Saraswathyamma CK, Thulaseedharan A. Identification of DNA polymorphism within the clones of Hevea brasiliensis (Muell.) Arg. using RAPD analysis. Indian J Nat Rubb Res, 2002, 15(3): 172–181. [11] Feng SP, Li WG, Huang HS, Wang JY, Wu YT. Develop-ment, application and cross-species/genera transferability of EST-SSR makers for rubber tree (Hevea brasiliensis). Mol Breed, 2009, 23: 85–97. [12] 张军, 张天真. 棉花微卫星标记的PAGE/银染快速检测. 棉花学报, 2000, 12(5): 267–269. [13] 施季森, 童春发. 树木遗传图谱构建和QTL定位统计分析. 北京: 科学出版社 2006, 82–86. [14] Hemma TM, Weedon NF, Manganaris, AG, Lawson DM. Molecular marker linkage map for apple. J Hered, 1994, 85(1): 4–11. [15] Testolin R, Huang WG, Lain O, Messina R, Vecchione A, Cipriani G. A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theor Appl Genet, 2001, 103: 30–36. [16] Porceddu A, Albertini E, Barcaccia G, Falistocco E, Fal-cinelli M. Linkage mapping in apomictie and sexual Ken-tucky bluegrass (Poa pratensis L.) genotypes using a two-way pseudo-testcross strategy based on AFLP and SAMPL markers. Theor Appl Genet, 2002, 104(2-3): 273–280. [17] 张德强, 张志毅, 宋婉等. 毛白杨遗传作图最适分离群体的选择. 北京林业大学学报, 2003, 25(4): 21–25. [18] 窦美安, 郭森元, 叶应福. 中规模推广级橡胶抗寒高产品种IAN873的引种利用研究. 热带作物学报, 2002, 23(3): 21–26. [19] 黄华孙. 中国橡胶树育种五十年. 北京: 中国农业出版社, 2005, 170–190. [20] 高妍, 韩明玉, 赵彩平, 宋健. 桃分子连锁图谱的构建. 果树学报, 2008, 25(4): 478–484. [21] Temesgen B, Brown GR, Harry DE, Kinlaw CS, Sewell MM, Neale DB. Genetic mapping of expressed sequence tag polymorphism (ESTP) markers in loblolly pine (Pinus taeda L.). Theor Appl Genet, 2001, 102(5): 664–675. [22] Nikaido AM, Ujino T, Iwata H, Yoshimura K, Yoshimura H, Suyama Y, Murai M, Nagasaka K, Tsumura Y. AFLP and CAPS linkage maps of Cryptomeria japonica. Theor Appl Genet, 2000, 100(6): 825–831. [23] Xu SJ, Singh RJ, Hymowitz T. Establishment of a cyto-genetic map soybean: progress and prospective. Soybean Genet Newslett, 1997, 24: 121–122. [24] Kinishita T. Report of the committee on gene mbolization, nomenclature and linkage group. Rice Genet Newslett, 1991, 8: 2–37. [25] Tzeng TH, Lyngholm LK, Ford CF, Bronson CR. A re-striction fragment length polymorphism map and electro-
Outlines

/