[an error occurred while processing this directive]
en

Association between chicken A-FABP gene polymorphisms and growth and body composition traits

Expand
  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2010-04-01

  Revised date: 2010-05-24

  Online published: 2011-02-25

Abstract

This experiment was designed to study the effects of polymorphism of A-FABP gene on growth and body composition traits in chicken. The 10th generation broiler population, derived from the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) was used. Polymorphism among individuals was detected by DNA sequencing, PCR-RFLP, PCR-LP, and DHPLC. Linkage disequilibrium analysis for eight SNPs was performed, and five htSNPs were selected to construct haplotypes. The association analysis between the individual SNPs and haplotypes and growth and body composition traits were investigated, respectively. The results showed that there were consistently significant effects on muscle stomach weight (MSW) and percentage of muscle stomach (MSW/BW) (P<0.05) in the seven SNPs (except for SNP 5) and haplotypes, but no significant effect on any other trait (P>0.05). Due to no evidence on effects of A-FABP for digestion system in other species, future experiments need to be developed to confirm whether A-FABP could be a major gene of MSW and MSW/BW traits in broiler chicken.

Cite this article

WANG Qi-Gui, GUAN Tian-Zhu, WANG Shou-Zhi, ZHANG Hui, WANG Zhi-Feng, LI Hui . Association between chicken A-FABP gene polymorphisms and growth and body composition traits[J]. Hereditas(Beijing), 2011 , 33(2) : 153 -162 . DOI: 10.3724/SP.J.1005.2011.00153

References

[1] Li H, Deeb N, Zhou H, Mitchell AD, Ashwell CM, La-mont SJ. Chicken quantitative trait loci for growth and body composition associated with transforming growth factor-β genes. Poul Sci, 2003, 82(3): 347-356.

[2] Li H, Deeb N, Zhou H, Ashwell CM, Lamont SJ. Chicken quantitative trait loci for growth and body composition associated with the very low density apolipoprotein-II gene. Poult Sci, 2005, 84(5): 697-703.

[3] Veerkamp JH, Maatman RG. Cytoplasmic fatty acid-binding proteins: their structure and genes. Prog Lipid Res, 1995, 34(1): 17-52.

[4] Veerkamp JH, Peeters RA, Maatman RG. Structural and functional features of different types of cytoplasmic fatty acid-binding proteins. Biochim Biophys Acta, 1991, 1081(1): 1-24.

[5] Coe NR, Simpson MA, Bernlohr DA. Targeted disruption of the adipocyte lipid-binding protein (aP2 protein) gene impairs fat cell lipolysis and increases cellular fatty acid levels. J Lipid Res, 1999, 40(5): 967-972.

[6] Hotamisligil GS, Johnson RS, Distel RJ, Ellis R, Papaio-annou VE, Spiegelman BM. Uncoupling of obesity from insulin resistance through a targeted mutation in aP2, the adipocyte fatty acid binding protein. Science, 1996, 274(5291): 1377-1379.

[7] Uysal KT, Scheja L, Wiesbrock SM, Bonner-Weir S, Ho-tamisligil GS. Hotamisligil. Improved glucose and lipid metabolism in genetically obese mice lacking aP2. Endocrinology, 2000, 141(9): 3388-3396.

[8] Wang QG, Li H, Li N, Gu ZL, Wang YX. Cloning and characterization of chicken adipocyte fatty acid binding protein gene. Anim Biotechnol, 2004, 15(2): 121-132.

[9] Shi H, Wang Q, Zhang Q, Leng L, Li H. Tissue expression characterization of chicken adipocyte fatty acid-binding protein and its expression difference between fat and lean birds in abdominal fat tissue. Poult Sci, 2010, 89(2): 197-202.

[10] Li WJ, Li HB, Chen JL, Zhao GP, Zheng MQ, Wen J. Gene expression of heart-and adipocyte-fatty acid-binding protein and correlation with intramuscular fat in Chinese chickens. Anim Biotechnol, 2008, 19(3): 190-194.

[11] 王启贵, 李宁, 邓学梅, 顾志良, 刘兆良, 李辉, 陈志强, 连正兴, 吴常信. 鸡脂肪酸结合蛋白基因的克隆和测序分析. 遗传学报, 2002, 29(2): 115-118.

[12] Wang Q, Li H, Li N, Leng L, Wang Y, Tang Z. Identifica-tion of single nucleotide polymorphism of adipocyte fatty acid-binding protein gene and its association with fatness traits in the chicken. Pouly Sci, 2006, 85(3): 429-434.

[13] 叶满红, 曹红鹤, 文杰, 李宏滨, 陈继兰, 赵桂萍. 北京油鸡和矮脚鸡心脏型、脂肪型脂肪酸结合蛋白基因多态性的研究. 畜牧兽医学报, 2003, 34(5): 422-426.

[14] 陈宽维, 章双杰, 屠云洁, 汤青萍, 高玉时, 李慧芳. A-FABP在不同鸡种中遗传多态性分析. 畜牧兽医学报, 2006, 37(11): 1114-1117.

[15] 罗桂芬, 陈继兰, 文杰, 赵桂苹, 郑麦青, 孙世铎. 鸡A-FABP基因多态性分析及其与脂肪性状的相关研究. 遗传, 2006, 28(1): 39-42.

[16] 屠云洁, 陈宽维, 章双杰, 汤清萍, 高玉时, 李慧芳, 苏一军. 3个鸡种A-FABP基因单核苷酸多态性的研究. 扬州大学学报(农业与生命科学版), 2004, 25(4): 53-55.

[17] 刘琛, 文杰, 赵桂苹, 郑麦青, 陈继兰. A-FABP与AMPD 1基因SNP位点对北京油鸡部分肉质性状的组合效应分析. 畜牧兽医学报, 2009, 40(10): 1555-1559.

[18] Wang QG, Guan TZ, Li H, Bernlohr DA. A novel poly-morphism in the chicken adipocyte fatty acid-binding protein gene (FABP4) that alters ligand-binding and correlates with fatness. Comp Biochem Physiol B Biochem Mol Biol, 2009, 154(3): 298-302.

[19] 初丽丽. 鸡FABPs基因多态性与生长和体组成性状的相关研究

[学位论文].哈尔滨: 东北农业大学, 2008.

[20] Barrett JC, Fry B, Maller J, Daly MJ. Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics, 2005, 21(2): 263-265.

[21] Carlson CS, Eberle MA, Rieder MJ, Yi Q, Kruglyak L, Nickerson DA. Selecting a maximally informative set of single-nucleotide polymorphisms for association analyses using linkage disequilibrium. Am J Hum Genet, 2004, 74(1): 106-120.

[22] Gabriel SB, Schaffner SF, Nguyen H, Moore JM, Roy J, Blumenstiel B, Higgins J, DeFelice M, Lochner A, Faggart M, Liu-Cordero SN , Rotimi C, Adeyemo A, Cooper R, Ward R, Lander ES, Daly MJ, Altshuler D. The structure of haplotype blocks in the human geno
Outlines

/