Circular chromosome conformation capture (4C) can be used to analyze the high-resolution interaction map of cis-regulatory elements in genome-wide studies. In this study, we optimized the condition of PCR with the mimical 4C sample in order to establish a specific and efficient assay, which was validated practically. This 4C-clone screening assay can be used as the quality control in the application of 4C assay.
TIAN Jing, CHEN Na, DIAO Zhi-Hu, CHEN Hui-Feng
. The quality control of 4C-clone screening assay[J]. Hereditas(Beijing), 2011
, 33(4)
: 404
-410
.
DOI: 10.3724/SP.J.1005.2011.00404
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