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Applications of DNA transposons to the study of gene function in mice

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  • State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005, China

Received date: 2010-11-16

  Revised date: 2011-01-17

  Online published: 2011-05-25

Abstract

In the past decade, transposon-mediated insertional mutagenesis has been widely used in mammalian molecular genetics. As a convenient and efficient tool for genetic manipulation, transposon has played an important role in making transgenic animal models, performing gene therapy, and annotating gene function at the cellular level and by animal studies in vivo. This review focuses on the structure, function and latest research progress of DNA transposons applied in mouse genetics.

Cite this article

LIU Lin, ZHANG Mei-Li, HUANG Yue . Applications of DNA transposons to the study of gene function in mice[J]. Hereditas(Beijing), 2011 , 33(5) : 485 -493 . DOI: 10.3724/SP.J.1005.2011.00485

References

[1] McClintock B. The origin and behavior of mutable loci in maize. Proc Natl Acad Sci USA, 1950, 36(6): 344-355.
[2] Ivics Z, Hackett PB, Plasterk RH, Izsvák Z. Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell, 1997, 91(4): 501-510.
[3] Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell, 2005, 122(3): 473-483.
[4] Kawakami K, Noda T. Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells. Genetics, 2004, 166(2): 895-899.
[5] Lewin B. Genes Ⅸ. Boston, Toronto, London, Singapore: Jones and Bartlett Publishers, 2008.
[6] Izsvák Z, Ivics Z, Plasterk RH. Sleeping Beauty, a wide host-range transposon vector for genetic transforma-tion in vertebrates. J Mol Biol, 2000, 302(1): 93-102.
[7] Zayed H, Izsvák Z, Walisko O, Ivics Z. Development of hyperactive Sleeping Beauty transposon vectors by mutational analysis. Mol Ther, 2004, 9(2): 292-304.
[8] Suster ML, Sumiyama K, Kawakami K. Transposon-mediated BAC transgenesis in zebrafish and mice. BMC Genomics, 2009, 10: 477.
[9] Geurts AM, Collier LS, Geurts JL, Oseth LL, Bell ML, Mu D, Lucito R, Godbout SA, Green LE, Lowe SW, Hirsch BA, Leinwand LA, Largaespada DA. Gene muta-tions and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concate-mers. PLoS Genet, 2006, 2(9): e156.
[10] Mátés L, Chuah MK, Belay E, Jerchow B, Manoj N, Acosta-Sanchez A, Grzela DP, Schmitt A, Becker K, Matrai J, Ma L, Samara-Kuko E, Gysemans C, Pryputniewicz D, Miskey C, Fletcher B, VandenDriessche T, Ivics Z, Izsvák Z. Molecular evolution of a hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet, 2009, 41(6): 753-761.
[11] Cadiñanos J, Bradley A. Generation of an inducible and optimized piggyBac transposon system. Nucleic Acids Res, 2007, 35(12): e87.
[12] Ivics Z, Li MA, Mátés L, Boeke JD, Nagy A, Bradley A, Izsvák Z. Transposon-mediated gene manipulation in vertebrates. Nat Methods, 2009, 6(6): 415-422.
[13] VandenDriessche T, Ivics Z, Izsvák Z, Chuah MKL. Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells. Blood, 2009, 114(8): 1461-1468.
[14] Dupuy AJ. Current applications of transposons in mouse genetics. Methods Enzymol, 2010, 477: 53-70.
[15] Kokubu C, Horie K, Abe K, Ikeda R, Mizuno S, Uno Y, Ogiwara S, Ohtsuka M, Isotani A, Okabe M, Imai K, Takeda J. A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice. Nat Genet, 2009, 41(8): 946-952.
[16] Keng VW, Yae K, Hayakawa T, Mizuno S, Uno Y, Yusa K, Kokubu C, Kinoshita T, Akagi K, Jenkins NA, Copeland NG, Horie K, Takeda J. Region-specific saturation germ-line mutagenesis in mice using the Sleeping Beauty transposon system. Nat Methods, 2005, 2(10): 763-769.
[17] Kile BT, Hilton DJ. The art and design of genetic screens: mouse. Nat Rev Genet, 2005, 6(7): 557-567.
[18] Nagy A, Gertsenstein M, Vintersten K, Behringer R. Manipulating the Mouse Embryo: A Laboratory Manual. 3rd ed. NY: Cold Spring Harbor Laboratory, 2003.
[19] Henikoff S. Conspiracy of silence among repeated trans-genes. Bioessays, 1998, 20(7): 532-535.
[20] Dupuy AJ, Clark K, Carlson CM, Fritz S, Davidson AE, Markley KM, Finley K, Fletcher CF, Ekker SC, Hackett PB, Horn S, Largaespada DA. Mammalian germ-line transgenesis by transposition. Proc Natl Acad Sci USA, 200
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