In order to study the potential gene function of ovine EST-SSR markers, nine original EST of Ovine Skin Derived polymorphic EST-SSR loci, which were developed in an early study by our lab, were ontology annotated and Electro localized. The results revealed that the original ESTs of the six loci had high homology with known genes and three of them probably played an important role in wool traits. Compared with its cDNA library, 8 loci were located on chromosomes of cattle. The homology of chromosomes between cattle and sheep was estimated based on the similarity coefficients calcu-lated by positioning markers. Additionally, NJ clustering tree was establishedto serve for electro localization of ovine EST-SSR markers. Finally, 8 EST-SSR markers were successfully positioned on ovine chromosomes. The results from this study not only provide references for further studies on genetic mapping, in silico cloning of key genes for wool traits, but also are helpful to the researchs of chromosome evolution in animal.
WANG Zun-Bao, DIAO Zong-Qing, TU Feng, TUN Hong-Bin, BAN Qian, LIANG Yao-Wei, ZHENG Wei
. The gene ontology and electro localization of ovine skin derived EST-SSR markers[J]. Hereditas(Beijing), 2011
, 33(7)
: 731
-737
.
DOI: 10.3724/SP.J.1005.2011.00731
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