PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Therefore, we used PRDX6 as an important candidate gene for meat quality according to its physiological and biochemical function. Partial coding sequence of porcine PRDX6 was isolated and two potenial SNPs, one at 417 bp (C/T) and the other at 423 bp (A/G), were found in the fourth exon by comparison of the obtained sequence from different pig breeds. In order to explore the relationship between PRDX6 polymorphism and meat quality, genetic variation and trait association of these two SNPs were separately performed in 6 purebred pig population and 247 F2 “Large White × Meishan” resource population by pyrosequencing. The results showed that allele C was predominant in western pig breeds, while allele T was predominant in Chinese indigenous breeds at 417 bp (C/T). This SNP was significantly associated with the intramuscular fat and water moisture (P<0.05). The A/G mutation at 423 bp was significantly associated with drip water rate, water holding capacity, intramuscular fat, and water moisture (P<0.05). Allele A was predominant in western pig breeds, while al-lele G was predominant in Chinese indigenous breeds. These two SNPs were likely to be important markers affect-ing meat quality traits (especially the muscle tenderness).
LIU Yang, TUN Wang-Jun, ZUO Bei, LIN Zhu-Jing, XIONG Yuan-Chu
. Polymorphism in coding region of pig PRDX6 gene and its genetic effects analysis[J]. Hereditas(Beijing), 2011
, 33(7)
: 743
-748
.
DOI: 10.3724/SP.J.1005.2011.00743
[1] Rhee SG, Kang SW, Chang TS, Jeong W, Kim K. Peroxire-doxin, a novel family of peroxidases. IUBMB Life, 2001, 52(1-2): 35-41.
[2] Manevich Y, Fisher AB. Peroxiredoxin 6, a 1-Cys peroxnre-doxin, functions in antioxidant defense and lung phospholipid metabolism. Free Radic Biol Med, 2005, 38(11): 1422-2432.
[3] Chen JW, Dodia C, Feinstein SI, Jain MK, Fisher AB. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione per-oxidase and phospholipase A2 activities. J Biol Chem, 2000, 275(37): 28421-28427.
[4] Koohmaraie M. Biochemical factors regulating the toughen-ing and tenderization processes of meat. Meat Sci, 1996, 43(S1): 193-201.
[5] Lametsch R, Karlsson A, Rosenvold K, Andersen HJ, Roep-storff P, Bendixen E. Postmortem proteome changes of pro-cine muscle related to tenderness. J Agric Food Chem, 2003, 51(24): 6992-6997.
[6] Hamza A. Homology modeling and docking mechanism of the mercaptosuccinate and methotrexate to P. falciparum 1-Cys peroxiredoxin: a preliminary molecular study. J Biomol Struct Dyn, 2002, 20(1): 7-20.
[7] Wang Y, Manevich Y, Feinstein SI, Fisher AB. Adenovirus-mediated transfer of the 1-cys peroxiredoxin gene to mouse lung protects against hyperoxic injury. Am J Physiol Lung Cell Mol Physiol, 2004, 286(6): L1188-L1193.
[8] Fisher AB, Dodia C. Role of phospholipase A2 enzymes in degradation of dipalmitoyl phosphatidyl choline by granular pneumocytes. J Lipid Res, 1996, 37(5): 1057-1064.
[9] Fisher AB, Dodia C. Lysosomal-type PLA2 and turnover of alveolar DPPC. Am J Physiol Lung Cell Mol Physiol, 2001, 280(4): L748-L754.
[10] Jia X, Veiseth-Kent E, Grove H, Kuziora P, Aass L, Hildrum KI, Hollung K. Peroxiredoxin-6—a potential protein marker for meat tenderness in bovine longissimus thoracis muscle. J Anim Sci, 2009, 87(7): 2391-2399.
[11] Xu YJ, Jin ML, Wang LJ, Zhang AD, Zuo B, Xu DQ, Ren ZQ, Lei MG, Mo XY, Li FE, Zheng R, Deng CY, Xiong YZ. Differential proteome analysis of porcine skeletal muscles between Meishan and Large White. J Anim Sci, 2009, 87(8): 2519-2527.
[12] 熊远著, 邓昌彦. 种猪测定原理与方法. 北京: 中国农业出版社, 1999: 57-118.
[13] 闫晨霞, 赵钢涛, 杨凡, 丁媛媛, 白旭朋, 许景峰. 焦磷酸测序法检测外周血白细胞乙醛脱氢酶基因多态性. 解放军药学学报, 2010, 26(1): 1-3.
[14] Liu BH. Statistical genomics: linkage, mapping, and QTL analysis. Boca Rato: CRC Press LLC, 1998.
[15] 杨昭庆, 洪坤学. 单核苷酸多态性的研究进展. 国外医学(遗传学分册), 2000, 23(1): 4-8.
[16] 包广宇, 高春芳, 孔宪涛. 单核苷酸多态性的研究进展及其应用. 国外医学(遗传学分册), 2003, 26(1): 7-10.
[17] Kimchi-Sarfaty C, Oh JM, Kim IW, Sauna ZE, Calcagno AM, Ambudkar SV, Gottesman MM. A “silent” polymorphism in the MDR1 gene changes substrate specificity. Science, 2007, 315(5811): 525-528.
[18] Manevich Y, Sweitzer T, Pak JH, Feinstein SI, Muzykantov V, Fisher AB. 1-Cys peroxiredoxin over-expression protects cell against phospholipids peroxidation-mediated membrane dam-age. Proc Natl Acad Sci USA, 2002, 99(18): 11599-11604.
[19] Leyens G, Donnay I, Knoops B. Cloning of bovine peroxire-doxins-gene expression in bovine tissues and amino acid sequence comparison with rat, mouse and primate peroxire-doxins. Comp Biochem Physiol B Biochem Mol Biol, 2003, 136(4): 943-955.
