PEG1 affects animal embryo growth and maternal behavior. The paternal allele-specific expression of PEG1 was reported in most animal species, but the expressive pattern of PEG1 was not clear in piglets born. In order to investigate the imprinting pattern of PEG1 in pig, 166 samples were used to SNP of PEG1 from Landrace, Yorkshire, and Lantang breeds by PCR-SSCP. Single nucleotide polymorphism (SNP) of PEG1 in exon 12 was identfied. The genotype frequency and the expressive pattern were anslyzed in swine by RT-PCR-RFLP/SSCP. One SNP (a G→A transition) was identified in exon 12 of PEG1. Maternal expression of PEG1 exon 12 was observed in all major organs (stomach, thymus, pancreas, spleen, lung, muscle, liver, tongue, kidney, brain, bladder, and heart) and placenta of three heterozygous pigs. PEG1 was paternally expressed and maternally imprinted in swine.
LI Chi, GU Tong, TUN Tong-Shan, ZHANG Shou-Quan
. Studies on SNP and genomic imprinting of the PEG1 gene in swine[J]. Hereditas(Beijing), 2011
, 33(7)
: 738
-742
.
DOI: 10.3724/SP.J.1005.2011.00738
[1] Li T, Vu TH, Lee KO, Yang YW, Nguynen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR. An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa. Biol Chem, 2002, 277(16): 13518-13527.
[2] Beechey CV, Cattanach BM. Mouse imprinting. http://www.mgu.har.mrc.ac.uk. 2000.
[3] Matsumoto S, Katoh M, Oshimura M, Nakanishi H, Kodani M, Nakamoto M, Shigeoka Y, Shimizu E, Suda T, Okabe T, Watanabe A, Igishi T. Loss of imprinting of PEG1/MEST in lung cancer cell lines. Oncol Rep, 2004, 12(6): 1273-1278.
[4] Xu CC, Su LJ, Zhou QY, Li CC, Zhao SH. Imprinting analysis of the porcine MEST gene in 75 and 90 day placentas and prenatal tissues. Acta Biochim Biophys Sin, 2007, 39(8): 633-639.
[5] Barlow DP, Stöger R, Herrmann BG, Saito K, Schweifer N. The mouse insulin-like growth factor type-2 receptor is imprinted and closely linked to the Tme locus. Nature, 1991, 349(6304): 84-87.
[6] Kalscheuer VM, Mariman EC, Schepens MT, Rehder H, Ropers HH. The insulin-like growth factor type-2 receptor gene is imprinted in the mouse but not in humans. Nat Genet, 1993, 5(1): 74-78.
[7] Nolan CM, Killian JK, Petitte JN, Jirtle RL. Imprint status of M6P/IGF2R and IGF2 in chickens. Dev Genes Evol, 2001, 211(4): 179-183.
[8] Pedersen IS, Dervan P, McGoldrick A, Harrison M, Ponchel F, Speirs V, Isaacs JD, Gorey T, McCann A. Promoter switch: a novel mechanism causing biallelic PEG1/MEST expression in invasive breast cancer. Hum Mol Genet, 2002, 11(12): 1449-1453.
[9] Riesewijk AM, Blagitko N, Schinzel AA, Hu LD, Schulz U, Hamel BC, Ropers HH, Kalscheuer VM. Evidence against a major role of PEG1/MEST in Silver-Russell syndrome. Eur J Hum Genet, 1998, 6(2): 114-120.
[10] Lefebvre L, Viville S, Barton SC, Ishino F, Surani MA. Genomic structure and parent-of-origin-specific methylation of Peg1. Hum Mol Genet, 1997, 6(11): 1907-1915.
[11] Nishita Y, Sado T, Yoshida I, Takagi N. Effect of CpG methylation on expression of the mouse imprinted gene Mest. Gene, 1999, 226(2): 199-209.
