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TSA improve transgenic porcine cloned embryo development and transgene expression

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  • College of Life Science, Northeast Agricultural University of China, Harbin 150030, China

Received date: 2010-10-15

  Revised date: 2010-12-21

  Online published: 2011-07-25

Abstract

Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. Histone modification associated with epigenetics can directly influence the embryo development and transgene expression. Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. TSA prevents the chromatin structure from being condensed, so that transcription factor could binds to DNA sequence easily and enhance transgene expression. Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. In summary, TSA can significantly improve porcine reconstructed embryo development and transgene expression.

Cite this article

KONG Qiang-Ran, SHU Jiang, HUANG Bei, HUAN Yan-Jun, WANG Feng, DAN Yong-Gan, LIU Zhong-Feng, WU Mei-Ling, LIU Zhong-Hua . TSA improve transgenic porcine cloned embryo development and transgene expression[J]. Hereditas(Beijing), 2011 , 33(7) : 749 -756 . DOI: 10.3724/SP.J.1005.2011.00749

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