There are many pleiotropic genes playing key roles in regulating both vegetative growth and reproductive development in plants. A dwarf mutant of rice with deformed flowers, named as ddf1, was identified from indica rice breeding lines. Genetic analysis indicated that ddf1 was resulted from the recessive mutation of a single gene, temporarily named as DDF1. This result suggested that DDF1 is a pleiotropic gene, which controls both vegetative growth and reproductive development in rice. To map this gene, an F2 population was developed by crossing the ddf1 heterozygote with the tropical japonica rice variety DZ60. By means of bulked segregant analysis and small population-based linkage analysis using the published RM-series rice SSR markers, DDF1 was preliminarily mapped in a region between markers RM588 and RM587 on chromosome 6 with the ge-netic distances of 3.8 cM and 2.4 cM to the two markers, respectively. By developing new SSR markers in this interval ac-cording to the published rice genome sequence, we further mapped DDF1 in a 165 kb interval. The results will fa-cilitate cloning of DDF1.
Sheng-Ping, DUAN Yuan-Lin, CHEN Zhi-Wei, GUAN Hua-Zhong, WANG Chuan-Lei, ZHENG Lei-Lei, ZHOU Yuan-Chang, WU Wei-Ren
. Genetic analysis and gene mapping of DDF1, a pleiotropic gene involving in both vegetable and reproductive growth in rice[J]. Hereditas(Beijing), 2011
, 33(12)
: 1374
-1379
.
DOI: 10.3724/SP.J.1005.2011.01374
[1] 罗琼, 王文明, 肖晗, 朱立煌, 周开达, 汪旭东, 王秀红. 一个新的水稻叶片和雌蕊发育异常突变体的遗传分析及其基因的分子标记定位. 科学通报, 2001, 46(15): 1277-1280.
[2] Yamaguchi T, Nagasawa N, Kawasaki S, Matsuoka M, Nagato Y, Hirano HY. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell, 2004, 16(2): 500-509.
[3] Smalle J, Kurepa J, Yang PZ, Emborg TJ, Babiychuk E, Kushnir S, Vierstra RD. The pleiotropic role of the 26S proteasome subunit RPN10 in Arabidopsis growth and development supports a substrate-specific function in abscisic acid signaling. Plant Cell, 2003, 15(4): 965-980.
[4] Wang H, Jones B, Li ZG, Frasse P, Delalande C, Regad F, Chaabouni S, Latché A, Pech JC, Bouzayen M. The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell, 2005, 17(10): 2676-2692.
[5] Radchuk R, Radchuk V, Weschke W, Borisjuk L, Weber H. Repressing the expression of the SUCROSE NONFERM ENTING-1-RELATED PROTEIN KINASE gene in pea embryo causes pleiotropic defects of maturation similar to an abscisic acid-insensitive phenotype. Plant Physiol, 2006, 140(1): 263-278.
[6] Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and or-ganless embryos. Plant J, 2009, 60(2): 227-241.
[7] Tian L, Chen ZJ. Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development. Proc Natl Acad Sci USA, 2001, 98(1): 200-205.
[8] Nelissen H, Clarke JH, Block MD, De Block S, Vander-haeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, van Li-jsebettens M. DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants. Plant Cell, 2003, 15(3): 639-654.
[9] Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL. Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis. Genetics, 2000, 156(3): 1363-1377.
[10] 秘彩莉, 刘旭, 张学勇. F-box蛋白质在植物生长发育中的功能. 遗传, 2006, 28(10): 1337-1342.
[11] González-Carranza GH, Rompa U, Peters JL, Bhatt AM, Wagstaff C, Stead AD, Roberts JA. HAWAIIAN SKIRT: An F-box gene that regulates organ fusion and growth in Arabidopsis. Plant Physiol, 2007, 144(3): 1370-1382.
[12] Deal RB, Kandasamy MK, McKinney EC, Meagher RB. The nuclear actin-related protein ARP6 is a pleiotropic developmental regulator required for the maintenance of FLOWERING LOCUS C expression and repres-sion of flowering in Arabidopsis. Plant Cell, 2005, 17(10): 2633-2646.
[13] Pei YX, Niu LF, Lu FL, Liu CY, Zhai JX, Kong XF, Cao XF. Mutations in the type II protein arginine methyltrans-ferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis. Plant Physiol, 2007, 144(4): 1913-1923.
[14] Yang J, Sardar H S, McGovern K R, Zhang YZ, Showalter AM. A lysine-rich arabinogalactan protein in Arabidopsis is essential for plant growth and development, including cell division and expansion. Plant J, 2007, 49(4): 629-640.
[15] Ge L, Chen H, Jiang JF, Zhao Y, Xu ML, Xu YY, Tan KH, Xu ZH, Chong K. Overexpression of OsRAA1 causes pleiotropic phenotypes in transgenic rice plants, including altered leaf, flower, and root development and root response to gravity. Plant Physiol, 2004, 135(3): 1502-1513.
[16] Xue WY, Xing YZ, Weng XY, Zhao Y, Tang WJ, Wang L, Zhou HJ, Yu SB, Xu CG, Li XH, Zhang QF. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761-767.
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