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Application of next generation sequencing techniques in plant transcriptome

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  • The Research and Development Laboratory for Plant Resources, Institute of Bontany, Chinese Acdemy of Sciences, Beijing 100093, China

Received date: 2010-11-16

  Revised date: 2011-08-24

  Online published: 2011-12-25

Abstract

With the development of DNA sequencing techniques, the next-generation sequencing (NGS) techniques with the characteristics of high-throughput and low cost have become the first choice for more and more researchers to carry out the biological researches. Among the next-generation sequencing techniques, the 454 sequencing platform is the first com-mercially available and relatively mature one and widely used in various fields of biological research. Taking 454 sequenc-ing platform as an example, we illustrate the advantages and disadvantages of NGS technical principles, review their appli-cations in plant transcriptome, and outlook their future development and applications in plant research field.

Cite this article

LIANG Ye, CHEN Shuang-Yan, LIU Gong-She . Application of next generation sequencing techniques in plant transcriptome[J]. Hereditas(Beijing), 2011 , 33(12) : 1317 -1326 . DOI: 10.3724/SP.J.1005.2011.01317

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