Experimental gene engineering is a laboratory course focusing on the molecular structure, expression pattern and biological function of genes. Providing our students with a solid knowledge base and correct ways to conduct research is very important for high-quality education of genetic engineering. Inspired by recent progresses in this field, we improved the experimental gene engineering course by adding more updated knowledge and technologies and emphasizing on the combination of teaching and research, with the aim of offering our students a good start in their scientific careers.
WU Yan-Hua, GUO Bin, LOU Hui-Ling, CUI Yu-Liang, GU Hui-Juan, QIAO Shou-Yi
. From gene cloning to expressional analysis——Practice and experience from educational reform of experimental gene engineering[J]. Hereditas(Beijing), 2012
, 34(2)
: 248
-252
.
DOI: 10.3724/SP.J.1005.2012.00248
[1] Jackson DA, Symons RH, Berg P. Biochemical method for inserting new genetic information into DNA of simian vi-rus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli. Proc Natl Acad Sci USA, 1972, 69(10): 2904-2909.
[2] 彭秀玲, 袁汉英. 基因工程实验技术. 长沙: 湖南科学技术出版社, 1997.
[3] 盛小禹. 基因工程实验技术教程. 上海: 复旦大学出版社, 1999.
[4] 邹克琴, 叶子弘, 李素芳. 改进《基因工程》教学效果的探讨. 中国科教创新导刊, 2007, (23): 51-53.
[5] Krüssel JS, Behr B, Milki AA, Hirchenhain J, Wen Y, Bielfeld P, Lake Polan M. Vascular endothelial growth factor (VEGF) mRNA splice variants are differentially expressed in human blastocysts. Mol Hum Reprod, 2001, 7(1): 57-63.
[6] 齐凤慧, 詹亚光. 《基因工程》实验教学改革的探索. 实验科学与技术, 2008, 6(1): 111-113.
[7] 许崇波, 迟彦, 高凤山. 基因工程实践教学改革的探索. 实验科学与技术, 2008, 6(4): 109-112.
