Tomato yellow leaf curl virus (TYLCV) is currently considered as one of the most devastating viruses in cultivated tomatoes (Solanum lycopersicum) worldwide. We reported here the development of a PCR-based method to quickly detect TYLCV using the primer pairs (TYLCV-F: 5'-ACG CAT GCC TCT AAT CCA GTG TA-3' and TYLCV-R: 5'-CCA ATA AGG CGT AAG CGT GTA GAC-3'), which was designed based on the genome sequence of TYLCV. A TYLCV-specific band of 543 bp was amplified from infected tomato plants. This protocol provides a rapid, reliable, and sensitive tool for molecular detection and identification of TYLCV in the industrial seedling and virus resistance breeding to facilitate safe and sustainable production of tomato.
LI Chang-Bao, CUI Yan-Ling, ZHANG Li-Ying, LI Chuan-You
. Molecular detection of tomato yellow leaf curl virus (TYLCV)[J]. Hereditas(Beijing), 2012
, 34(3)
: 366
-370
.
DOI: 10.3724/SP.J.1005.2012.00366
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