Most cells contain various transport vesicles that target to different destinations. The underlying molecular mechanisms are highly conserved in evolution. Sec1/Munc-18 (SM) proteins play an important role on regulating vesicle transport by interacting with soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) at each vesicle fusion sites. SM proteins interact with syntaxin, an important component in SNARE complex, to regulate the assembly of SNARE complex, and promote overall membrane fusion process together with SNARE complex. This review summaries new research progresses of structure and function of SM protein.
ZHAO Xiang, HAN Bao-Da, LI Li-Xin
. Function of SM protein in vesicle transport[J]. Hereditas(Beijing), 2012
, 34(4)
: 389
-400
.
DOI: 10.3724/SP.J.1005.2012.00389
[1] Wickner W, Schekman R. Membrane fusion. Nat Struct Mol Biol, 2008, 15(7): 658- 664.
[2] Bonifacino JS, Glick BS. The mechanisms of vesicle bud-ding and fusion. Cell, 2004, 116(2): 153-166.
[3] Cai HQ, Reinisch K, Ferro-Novick S. Coats, tethers, rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle. Dev Cell, 2007, 12(5): 671- 682.
[4] Südhof TC, Rothman JE. Membrane fusion: Grappling with SNARE and SM proteins. Science, 2009, 323(5913): 474-477.
[5] Robinson LJ, Martin TFJ. Docking and fusion in neurose-cretion. Curr Opin Cell Biol, 1998, 10(4): 483-492.
[6] Hanson PI, Roth R, Morisaki H, Jahn R, Heuser JE. Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/ deep-etch electron microscopy. Cell, 1997, 90(3): 523-535.
[7] Weber T, Zemelman BV, McNew JA, Westermann B, Gmachl M, Parlati F, Söllner TH, Rothman JE. SNAREpins: minimal machinery for membrane fusion. Cell, 1998, 92(6): 759-772.
[8] Brenner S. The genetics of Caenorhabditis elegans. Genetics, 1974, 77(1): 71-94.
[9] Novick P, Field C, Schekman R. Identification of 23 com-plementation groups required for post-translational events in the yeast secretory pathway. Cell, 1980, 21(1): 205-215.
[10] Hata Y, Slaughter CA, Südhof TC. Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin. Nature, 1993, 366(6453): 347-351.
[11] Bracher A, Weissenhorn W. Crystal structures of neuronal squid Sec1 implicate inter-domain hinge movement in the release of t-SNAREs. J Mol Biol, 2001, 306(1): 7-13.
[12] Bracher A, Weissenhorn W. Structural basis for the Golgi membrane recruitment of Sly1p by Sed5p. EMBO J, 2002, 21(22): 6114-6124.
[13] Carr CM, Grote E, Munson M, Hughson FM, Novick PJ. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J Cell Biol, 1999, 146(2): 333-344.
[14] Novick P, Schekman R. Secretion and cell-surface growth are blocked in a temperature- sensitive mutant of Sac-charomyces cerevisiae. Proc Natl Acad Sci USA, 1979, 76(4): 1858- 1862.
[15] Ossig R, Dascher C, Trepte HH, Schmitt HD, Gallwitz D. The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endo-plasmic reticulum-to-Golgi transport. Mol Cell Biol, 1991, 11(6): 2980-2993.
[16] Dascher C, Ossig R, Gallwitz D, Schmitt HD. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPT1, a member of the RAS superfamily. Mol Cell Biol, 1991, 11(2): 872-885.
[17] Lupashin VV, Waters MG. t-SNARE activation through transient interaction with a rab-like guanosine triphosphatase. Science, 1997, 276(5316): 1255-1258.
[18] Dulubova I, Yamaguchi T, Wang Y, Südhof TC, Rizo J. Vam3p structure reveals conserved and divergent properties of syntaxins. Nat Struct Biol, 2001, 8(3): 258-264.
[19] Banta LM, Vida TA, Herman PK, Emr SD. Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis. Mol Cell Biol, 1990, 10(9): 4638- 4649.
[20] Cowles CR, Emr SD, Horazdovsky BF. Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles. J Cell Sci, 1994, 107(12): 3449-3459.
[21] Bryant NJ, James DE. Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE com-plex formation. EMBO J, 2001, 20(13): 3380-3388.
[22] Webb GC, Hoedt M, Poole LJ, Jones EW. Genetic inter-actions between a pep7 mutation and the PEP12 and VPS45 genes: evidence for a novel SNARE component in transport between the Saccharomyces cerevisiae Golgi complex and endosome. Genetics, 1997, 147(2
