[1] Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bess-ieres P, Boland F, Brignell SC, Bron S, Bunai K, Chapuis J,. Essential Bacillus subtilis genes. Proc Natl Acad Sci USA, 2003, 100(8): 4678-4683.
[2] Koonin EV. Comparative genomics, minimal gene-sets and the last universal common ancestor. Nat Rev Mi-crobiol, 2003, 1(2): 127-136.
[3] Gil R, Silva FJ, Peretó J, Moya A. Determination of the core of a minimal bacterial gene set. Microbiol Mol Biol Rev, 2004, 68(3): 518-537.
[4] Hu WQ, Sillaots S, Lemieux S, Davison J, Kauffman S, Breton A, Linteau A, Xin CL, Bowman J, Becker J, Jiang B, Roemer T. Essential gene identification and drug target prioritization in Aspergillus fumigatus. PLoS Pathog, 2007, 3(3): e24.
[5] Haselbeck R, Wall D, Jiang B, Ketela T, Zyskind J, Bussey H, Foulkes JG, Roemer T. Comprehensive essential gene Identification as a platform for novel antiinfective drug discovery. Curr Pharm Des, 2002, 8(13): 1155-1172.
[6] Itaya M. An estimation of minimal genome size required for life. FEBS Lett, 1995, 362(3): 257-260.
[7] Hutchison CA III, Peterson SN, Gill SR, Cline RT, White O, Fraser CM, Smith HO, Venter1 JC. Global transposon mutagenesis and a minimal Mycoplasma genome. Science, 1999, 286(5447): 2165-2169.
[8] Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA III, Smith HO, Venter JC. Essential genes of a minimal bacterium. Proc Natl Acad Sci USA, 2006, 103(2): 425-430.
[9] Ji YD, Zhang B, van Horn SF, Warren P, Woodnutt G, Burnham MKR, Rosenberg M. Identification of critical staphylococcal genes using conditional phenotypes gener-ated by antisense RNA. Science, 2001, 293(5538): 2266-2269.
[10] Forsyth RA, Haselbeck RJ, Ohlsen KL, Yamamoto RT, Xu H, Trawick JD, Wall D, Wang LS, Brown-Driver V, Froelich JM, C KG, King P, McCarthy M, Malone C, Misiner B, Robbins D, Tan ZH, Zhu ZY, Carr G, Mosca DA, Zamudio C, Foulkes JG, Zyskind JW. A genome-wide strategy for the identification of essential genes in Staphylo-coccus aureus. Mol Microbiol, 2002, 43(6): 1387-1400.
[11] Ko KS, Lee JY, Song JH, Baek JY, Oh WS, Chun JK, Yoon HS. Screening of Essential genes in Staphylococcus aureus N315 using comparative genomics and allelic replacement mutagenesis. J Microbiol Biotechnol, 2006, 16(4): 623-632.
[12] Chaudhuri RR, Allen AG, Owen PJ, Shalom G, Stone K, Harrison M, Burgis TA, Lockyer M, Jorge GL, Foster SJ, Pleasance SJ, Peters SE, Maskell DJ, Charles IG. Comprehensive identification of essential Staphylococcus aureus genes using transposon-mediated differential hybridisation (TMDH). BMC Genomics, 2009, 10(1): 291.
[13] Salama NR, Shepherd B, Falkow S. Global transposon mutagenesis and essential gene analysis of Helico-bacter pylori. J Bacteriol, 2004, 186(23): 7926-7935.
[14] Akerley BJ, Rubin EJ, Novick VL, Amaya K, Judson N, Mekalanos JJ. A genome-scale analysis for identification of genes required for growth or survival of Haemo-philus influenzae. Proc Natl Acad Sci USA, 2002, 99(2): 966-971.
[15] Sassetti CM, Boyd DH, Rubin EJ. Genes required for mycobacterial growth defined by high density mutagenesis. Mol Microbiol, 2003, 48(1): 77-84.
[16] Gerdes SY, Scholle MD, Campbell JW, Balázsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabási AL, Oltvai ZN, Osterman AL. Experimental determination and system level analysis of essential genes in Escherichia coli MG1655. J Bacteriol, 2003, 185(19): 5673-5684.
[17] Knuth K, Niesalla H, Hueck CJ, Fuchs TM. Large-scale identification of essential Salmonella genes by trapping lethal inse
