The family members of PEX11 are key factors involved in regulation of peroxisome proliferation. Sixty-six PEX11p candidates of PEX11 gene family from26 representative fungal species were obtainedand analyzed by bioinformatic strategies. In most filamentous fungi, 2 or 3 potential PEX11ps were found, in contrast with 1 or 2 in yeast species. Com-pared with other fungal species, the Ascomycetes tend to have more PEX11ps, and even 5 in several individuals. The data of phylogenetic analysis and protein structure indicated that all of the PEX11ps were divided into 3groups: I, II, and III. The members of group I and groupIIIexisted in most species, while those in group II were found only in Pezizomycotina. By MEME analysis, 5-6 conserved motifs were found in each PEX11ps. Among them,motif 8 in C-terminal had the most conservation, indicating that this motif probably plays a key role in maintaining the proper functionof PEX11p.
ZHANG Xin, JIANG Hua, WANG Yan-Li, ZHANG Zhen, MAO Xue-Qin, CHAI Rong-Yao, QIU Hai-Ping, DU Xin-Fa, WA Jiao-Yu, SUN Guo-Chang
. Bioinformaticresearch of the family of PEX11, peroxisomeproliferous factor in fungus[J]. Hereditas(Beijing), 2012
, 34(5)
: 635
-646
.
DOI: 10.3724/SP.J.1005.2012.00635
[1] Veenhuis M, Salomons FA, van Der Klei IJ. Peroxisome biogenesis and degradation in yeast: a structure/function analysis. Microsc Res Tech, 2000, 51(6): 584-600.
[2] Spröte P, Brakhage AA, Hynes MJ. Contribution of peroxisomes to penicillin biosynthesis in Aspergillus nidulans. Eukaryot Cell, 2009, 8(3): 421-423.
[3] Rottensteiner H, Stein K, Sonnenhol E, Erdmann R. Conserved function of pex11p and the novel pex25p and pex27p in peroxisome biogenesis. Mol Biol Cell, 2003, 14(10): 4316-4328.
[4] Hayashi M, Nishimura M. Entering a new era of research on plant peroxisomes. Curr Opin Plant Biol, 2003, 6(6): 577-582.
[5] Orth T, Reumann S, Zhang XC, Fan JL, Wenzel D, Quan S, Hu JP. The PEROXIN11 protein family controls peroxisome proliferation in Arabidopsis. Plant Cell, 2007, 19(1): 333-350.
[6] Parsons M, Furuya T, Pal S, Kessler P. Biogenesis and function of peroxisomes and glycosomes. Mol Biochem Parasitol, 2001, 115(1): 19-28.
[7] Yan MD, Rayapuram N, Subramani S. The control of peroxisome number and size during division and proliferation. Curr Opin Cell Biol, 2005, 17(4): 376-383.
[8] Titorenko VI, Terlecky SR. Peroxisome metabolism and cellular aging. Traffic, 2011, 12(3): 252-259.
[9] Oku M, Sakai Y. Peroxisomes as dynamic organelles: autophagic degradation. FEBS J, 2010, 277(16): 3289-3294.
[10] Hu JP. Molecular basis of peroxisome division and proliferation in plants. Int Rev Cell Mol Biol, 2010, 279: 79-99.
[11] Muench DG, Mullen RT. Peroxisome dynamics in plant cells: a role for the cytoskeleton. Plant Sci, 2003, 164(3): 307-315.
[12] Huybrechts SJ, van Veldhoven PP, Brees C, Mannaerts GP, Los GV, Fransen M. Peroxisome dynamics in cultured mammalian cells. Traffic, 2009, 10(11): 1722-1733.
[13] Thoms S, Erdmann R. Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation. FEBS J, 2005, 272(20): 5169-5181.
[14] Kiel JAKW, Veenhuis M, van der Klei IJ. PEX genes in fungal genomes: common, rare or redundant. Traffic, 2006, 7(10): 1291-1303.
[15] Mullen RT, Flynn CR, Trelease RN. How are peroxisomes formed? The role of the endoplasmic reticulum and peroxins. Trends Plant Sci, 2001, 6(6): 256-261.
[16] Marshall PA, Krimkevich YI, Lark RH, Dyer JM, Veenhuis M, Goodman JM. Pmp27 promotes peroxisomal proliferation. J Cell Biol, 1995, 129(2): 345-355.
[17] Krikken AM, Veenhuis M, van der Klei IJ. Hansenula polymorpha pex11 cells are affected in peroxisome retention. FEBS J, 2009, 276(5): 1429-1439.
[18] Li X, Baumgart E, Dong GX, Morrell JC, Jimenez-Sanchez G, Valle D, Smith KD, Gould SJ. PEX11alpha is required for peroxisome proliferation in response to 4-phenylbutyrate but is dispensable for peroxisome proliferator-activated receptor alpha-mediated peroxisome proliferation. Mol Cell Biol, 2002, 22(23): 8226-8240.
[19] Kobayashi S, Tanaka A, Fujiki Y. Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis. Exp Cell Res, 2007, 313(8): 1675-1686.
[20] Koch J, Pranjic K, Huber A, Ellinger A, Hartig A, Kragler F, Brocard C. PEX11 family members are membrane elongation factors that coordinate peroxisome proliferation and maintenance. J Cell Sci, 2010, 123(Pt 19): 3389-3400.
[21] Mast FD, Fagarasanu A, Knoblach B, Rachubinski RA. Peroxisome biogenesis: something old, something new, something borrowed. Physiology (Bethesda), 2010, 25(6): 347-356.
[22] Kovalchuk A, Driessen AJ. Phylogenetic analysis of fungal ABC transporters. BMC Genomics, 2010, 11: 177
[23] James TY, Kauff F, Schoch CL, Matheny PB, Hofstetter V, Cox CJ, Celio G, Gueidan C, Fraker E, Miadlikowska J, Lumbsch HT, Rauhut A, Reeb V, Arnold AE, Amtoft A, Vilgalys R. Reconstructing the early evolution of fungi using a
