Cloning and functional analysis of SCTF-1 encoding a C2H2-type zinc finger protein from soybean

SONG Bing, WANG Pi-Wu, FU Yong-Ping, FAN Xu-Hong, XIA Hai-Feng, GAO-Wei, HONG Yang, WANG He, ZHANG Zhuo, MA Jian

doi:10.3724/SP.J.1005.2012.00749

[an error occurred while processing this directive]

Hereditas(Beijing) >

2012 , Vol. 34 >Issue 6: 749 - 756

DOI: https://doi.org/10.3724/SP.J.1005.2012.00749

Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean

Expand

1. College of Agriculture, Jilin Agricultural University, Changchun 130118, China 2. Jilin City Academy of Agricultural Sciences, Jilin City 132101, China 3. Soybean Research Center, Jilin Academy of Agricultural Sciences, Changchun 130033, China 4. Tonghua Academy of Agricultural Sciences, Tonghua 135000, China

Received date: 2012-02-09

Revised date: 2012-03-02

Online published: 2012-06-25

Fold

Abstract

The zinc finger protein is one of the proteins with finger-like domain. Some of them are transcription factors which play important role in plant growth and plant resistance to abiotic stresses. In this paper, a novel C₂H₂-type zinc finger protein gene SCTF-1 (GenBank accession number JQ692081) was isolated from soybean (Glycine max (L.) Merr.) This gene has a 699 bp ORF (open reading frame) with no in-tron and encodes a 24.9 kDa protein with 233 amino acids. Its isoelectric point (pI) is 8.33. The SCTF-1 protein contains two typical C₂H₂-type zinc finger domains. Both of them have highly conserved amino acid sequence-QALGGH which is a particular characteristic of plant. Transient expression of the GFP-SCTF-1 protein in onion epidermal cell showed that SCTF-1 was localized in cell nuclei. RT-PCR results showed that SCTF-1 gene was expressed with high levels in flowers and leaves in soybean, but low in roots and stems. The expression of SCTF-1 gene was strongly induced by low temperature in the soybean seedlings. Overexpression of SCTF-1 enhanced cold tolerance of transgenic tobacco (Nicotiana tabacum L.) compared to the control.

Key words： soybean; zinc finger protein; C₂H₂-type; SCTF-1 gene; subcellular localization; cold tolerance

Cite this article

SONG Bing, WANG Pi-Wu, FU Yong-Ping, FAN Xu-Hong, XIA Hai-Feng, GAO-Wei, HONG Yang, WANG He, ZHANG Zhuo, MA Jian . Cloning and functional analysis of SCTF-1 encoding a C₂H₂-type zinc finger protein from soybean[J]. Hereditas(Beijing), 2012 , 34(6) : 749 -756 . DOI: 10.3724/SP.J.1005.2012.00749

References

[1] Sakamoto H, Araki T, Meshi T, Iwabuchi M. Expression of a subset of the Arabidopsis Cys₂/His₂-type zinc-finger protein gene family under water stress. Gene, 2000, 248(1-2): 23-32.
[2] Sugano S, Kaminaka H, Rybka Z, Catala R, Salinas J, Matsui K, Ohme-Takagi M, Takatsuji H. Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia. Plant J, 2003, 36(6): 830-841.
[3] 郭书巧, 黄骥, 江燕, 张红生. 水稻C2H2型锌指蛋白基因RZF71的克隆与表达分析. 遗传, 2007, 29(5): 607-618.
[4] 郭书巧, 张红生. 水稻锌指蛋白基因RZF5和RZF71的克隆与功能分析
[学位论文]. 南京: 南京农业大学, 2006: 23-120.
[5] Kim JC, Lee SH, Cheong YH, Yoo CM, Lee SI, Chun HJ, Yun DJ, Hong JC, Lee SY, Lim CO, Cho MJ. A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant J, 2001, 25(3): 247-259.
[6] 邢浩然, 刘丽娟, 刘国振. 植物蛋白质的亚细胞定位研究进展. 华北农学报, 2006, 21(增刊): 1-6.
[7] 黄骥, 王建飞, 张红生. 植物C2H2 型锌指蛋白的结构与功能. 遗传, 2004, 26(3): 414-418.
[8] 刘海燕, 冯冬茹, 刘兵, 何炎明, 王宏斌, 王金发. 农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究. 热带亚热带植物学报, 2009, 17(3): 218-222.
[9] 黄骥, 张红生. TFⅢA型锌指蛋白及在提高植物耐逆性中的作用. 遗传, 2007, 29(8): 915-922.
[10] Dinkins RD, Pflipsen C, Collins GB. Expression and deletion analysis of an Arabidopsis SUPERMAN-like zinc finger gene. Plant Sci, 2003, 165(1): 33-41.
[11] Payne T, Johnson SD, Koltunow AM. KNUCKLES (KNU) encodes a C2H2 zinc-finger protein that regulates devel-opment of basal pattern elements of the Arabidopsis gy-noecium. Development, 2004, 131(15): 3737-3749.
[12] Nakagawa H, Jiang CJ, Sakakibara H, Kojima M, Honda I, Ajisaka H, Nishijima T, Koshioka M, Homma T, Mander LN, Takatsuji H. Overexpression of a petunia zinc-finger gene alters cytokinin metabolism and plant forms. Plant J, 2005, 41(4): 512-521.
[13] Huang F, Chi YJ, Meng QC, Gai JY, Yu DY. GmZFP1 encoding a single zinc finger protein is expressed with enhancement in reproductive organs and late seed development in soybean (Glycine max). Mol Biol Rep, 2006, 33(4): 279-285.
[14] Lippuner V, Cyert MS, Gasser CS. Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild-type yeast. J Biol Chem, 1996, 271(22): 12859-12866.
[15] Sakamoto H, Araki T, Meshi T, Iwabuchi M. Expression of a subset of the Arabidopsis Cys₂/His₂-type zinc-finger protein gene family under water stress. Gene, 2000, 248(1-2): 23-31.
[16] van Der Krol AR, van Poecke RM, Vorst OF, Voogt C, van Leeuwen W, Borst-Vrensen TW, Takatsuji H, van Der Plas LH. Developmental and wound-, cold-, desiccation-, ul-traviolet-B-stress-induced modulations in the expression of the petunia zinc finger transcription factor gene ZPT2-2. Plant Physiol, 1999, 121(4): 1153-1162.
[17] 白晶, 张必弦, 李新玲, 徐香玲. 野生大豆(Glycine soja) C2H2型锌指蛋白基因的克隆与序列分析. 大豆科学, 2009, 28(1): 21-25.

Options

Outlines

〈

〉

www.chinagene.cn
备案号：京ICP备09063187号

模态框（Modal）标题

Abstract

Cite this article

References