Generation of goat <em>H-FABP</em> overexpression transgenic mice by testicular injection

YIN Yan-Hui, SUN Min, CHEN Ting-Feng, ZHANG Ya-Ni, ZHU Cai-Ye, LI Wei, LI Bi-Chun

doi:10.3724/SP.J.1005.2012.00727

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Hereditas(Beijing) >

2012 , Vol. 34 >Issue 6: 727 - 735

DOI: https://doi.org/10.3724/SP.J.1005.2012.00727

Generation of goat H-FABP overexpression transgenic mice by testicular injection

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Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal Science and Tech-nology, Yangzhou University, Yangzhou 225009, China

Received date: 2011-12-28

Revised date: 2012-02-17

Online published: 2012-06-25

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Abstract

To explore the possibility of transgenic animals by testicular injection, the goat heart-type fatty acid binding protein (H-FABP) expression vector pEGFP-H-FABP was injected into the testis of 6 mice randomly by liposome mediated transfection. By detection of testis slice, sperm fluorescence and sperm DNA PCR, the exogenous gene was expressed in the parental mice. The exogenous gene was expressed at different levels in both the F₁ generation mice gave birthed by treated male mice and normal female mice and the F₂ generation mice generated by mating F₁ could be detected that the exogenous gene expressed at different levels with the positive rates of 4% and 30.23%, respectively. The results suggested that testicular injection, as an effective method to generate transgenic animal, could realize the stable integration of exogenous gene. The amelioration and maturity of testicular injection provides theoretical and practical significance in generation of transgenic animals and even in the animal trait improvement and breeding.

Key words： testicular injection; transgenic mice; heart-type fatty acid binding protein

Cite this article

YIN Yan-Hui, SUN Min, CHEN Ting-Feng, ZHANG Ya-Ni, ZHU Cai-Ye, LI Wei, LI Bi-Chun . Generation of goat H-FABP overexpression transgenic mice by testicular injection[J]. Hereditas(Beijing), 2012 , 34(6) : 727 -735 . DOI: 10.3724/SP.J.1005.2012.00727

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