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Genetic scanning on chromosome 8 loci for coronary heart disease

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  • 1. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China 2. Laboratory of Medical Genetics, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, China 3. Department of Respiratory Medicine, Shandong Provincial Thoracic Hospital, Jinan 250013, China 4. Department of Intensive Care Unit, Qilu Hospital of Shandong University, Jinan 250012, China

Received date: 2012-02-14

  Revised date: 2012-03-28

  Online published: 2012-08-25

Abstract

At present, genome-wide association study on coronary heart disease (CHD) has been carried out in several major medical research centers worldwide. Most studies of CHD susceptibility loci or regions focused on chromosome 1, 3, 9, 11 and 16, while studies on chromosome 8 are rare. To the best of our knowledge, the genome study on chromosome 8 about CHD in Chinese Han population has never been reported before. We aimed to identify CHD susceptibility loci or regions in the Chinese Han population. First, two separated DNA pooling samples were prepared from 156 CHD cases and 1000 normal controls. Then, a total of 13 microsatellite markers at an interval of 10 cM on chromosome 8 were selected for genetic scanning. Finally, the difference of allele frequency at each locus between two pooled samples was analyzed by Chi-square test. Significant differences were found between cases and controls at D8S264 (8p23.3-p23.2) and D8S285 (8q12.1) (both P<0.05). Therefore, 8p23.3-p23.2 and 8q12.1 are possible to be associated with CHD and further study is needed to screen susceptible genes around these regions.

Cite this article

JIANG Ting-Ting, CHEN Xing, LI Ting-Ting, ZHANG Feng-Guo, XIE Yi, ZHANG Jian-Ning, PENG Jie, LIU Tian-Jiao, CHEN Gang, GUO Yuan . Genetic scanning on chromosome 8 loci for coronary heart disease[J]. Hereditas(Beijing), 2012 , 34(8) : 1043 -1049 . DOI: 10.3724/SP.J.1005.2012.01043

References

[1] Marenberg ME, Risch N, Berkman LF, Floderus B, de Faire U. Genetic susceptibility to death from coronary heart disease in a study of twins. N Engl J Med, 1994, 330(15): 1041-1046.
[2] Engert JC, Lemire M, Faith J, Brisson D, Fujiwara TM, Roslin NM, Brewer CG, Montpetit A, Darmond-Zwaig C, Renaud Y, Doré C, Bailey SD, Verner A, Tremblay G, StPierre J, Bétard C, Platko J, Rioux JD, Morgan K, Hudson TJ, Gaudet D. Identification of a chromosome 8p locus for early-onset coronary heart disease in a French Canadian population. Eur J Hum Genet, 2008, 16(1): 105-114.
[3] Barcellos LF, Klitz W, Field LL, Tobias R, Bowcock AM, Wilson R, Nelson MP, Nagatomi J, Thomson G. Association mapping of disease loci, by use of a pooled DNA ge-nomic screen. Am J Hum Genet, 1997, 61(3): 734-747.
[4] Ma ZJ, Yi HG, Zhao Y, Chen F. Using two-stage case-control designs to study the genome-wide association. Chinese J Epidemiol, 2010, 31(10): 1184-1187.
[5] Bharti AR, Letendre SL, Patra KP, Vinetz JM, Smith DM. Malaria diagnosis by a polymerase chain reaction-based assay using a pooling strategy. Am J Trop Med Hyg, 2009, 81(5): 754-757.
[6] Bugeja MJ, Booth DR, Bennetts BH, Heard RN, Stewart GJ. An investigation of polymorphisms in the 4q1 3. 3-21. 1 CXC chemokine gene cluster for association with multiple sclerosis in Australians. Mult Scler, 2006, 12(6): 710-722.
[7] Matkovich SJ, Van Booven DJ, Hindes A, Kang MY, Druley TE, Vallania FL, Mitra RD, Reilly MP, Cappola TP, Dorn GW 2nd. Cardiac signaling genes exhibit unexpected sequence diversity in sporadic cardiomyopathy, revealing HSPB7 polymorphisms associated with disease. J Clin Invest, 2010, 120(1): 280-289.
[8] 王博, 张成, 林汝湘, 陈刚, 魏然, 朱海宁, 栾萌, 周鹏, 高春义. 山东省原发性高血压1号染色体基因扫描. 中国高血压杂志, 2008, 16(2): 277-282.
[9] Liu Y, Chen G, Norton N, Liu W, Zhu H, Zhou P, Luan M, Yang S, Chen X, Carroll L, Williams NM, O'Donovan MC, Kirov G, Owen MJ. Whole genome association study in a homogenous population in Shandong peninsula of China reveals JARID2 as a susceptibility gene for schizophrenia. J Biomed Biotechnol, 2009, 2009: 536918, doi: 10.1155/ 2009/536918.
[10] Chen X, Tang J, Liu Y, Luan M, An K, Zhang Y, Li FH, Zhou P, Liu WM, Liu JT, Chen G. Lack of association between NCAM1 and early onset schizophrenia in a family based study in Shandong peninsula of China. J Pe-diatric Genet, 2011, 1(1): 39-45.
[11] 谢毅, 郭媛, 陈刚, 李继福, 朱海宁, 魏然, 王博, 栾萌. 山东地区冠状动脉粥样硬化心脏病患者3号染色体基因扫描研究. 山东大学学报, 2009, 47(1): 56-60.
[12] Adeyemo A, Luke A, Cooper R, Wu X, Tayo B, Zhu X, Rotimi C, Bouzekri N, Ward R. A genome-wide scan for body mass index among Nigerian families. Obes Res, 2003, 11(2): 266-273.
[13] Johnson L, Luke A, Adeyemo A, Deng HW, Mitchell BD, Comuzzie AG, Cole SA, Blangero J, Perola M, Teare MD. Meta-analysis of five genome-wide linkage studies for body mass index reveals significant evidence for linkage to chromosome 8p. Int J Obes, 2005, 29(4): 413-419.
[14] Naoumova RP, Bonney SA, Eichenbaum-Voline S, Patel HN, Jones B, Jones EL, Amey J, Colilla S, Neuwirth CK, Allotey R, Seed M, Betteridge DJ, Galton DJ, Cox NJ, Bell GI, Scott J, Shoulders CC. Confirmed locus on chromosome 11p and candidate loci on 6q and 8p for the triglyceride and cholesterol traits of combined hyperlipi-demia. Arterioscler Thromb Vasc Biol, 2003, 23(11): 2070-2077.
[15] Ling H, Waterworth DM, Stirnadel HA, Pollin TI, Barter PJ, Kesäniemi YA, Mahley RW, McPherson R, Waeber G, Bersot TP, Cohen JC, Grundy SM, Mooser VE, Mitchell BD. Genome-wide linkage and association analyses to identify genes influencing adiponectin levels: the GEMS Study. Obesity, 2009, 17(4): 737-744.
[16] Komura N, Kihara S, Sonoda M, Kumada M, Fujita K, Hiuge A, Okada T, Nakagawa Y, Tamba S, Kuroda Y, Ha-yashi N, Sumitsuji S, Kawamoto T, Matsumoto S, Ouchi N, Arita Y, Okamoto Y, Shimomura I
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