As a means of visual function testing and visual related mutants screening, the optokinetic response (OKR) and the optomotor response (OMR) behaviour tests are simple and effective tools for visual functional testing, which have been widely used in studying zebrafish larvae. However, adult zebrafish OKR analysis method is rarely reported. In this study, the methods of inducing adult zebrafish OKR behaviour, as well as tracking the movement of eyes using Pattern Match approaches, are presented. The quantitative measurement of the adult zebrafish OKR behaviour was successfully established. Using these methods, the binocular vision area was found to make a certain contribution to OKR behaviour. Moreover, the monocular vision of adult zebrafish showed a certain degree of directional sensitivity to moving gratings. Such approaches can also be applied to the zebrafish larvae OKR. The abnormal OKR behaviour phenomenon of period1b mutant larvae fish was detected.
HUANG Yu-Bin, ZOU Su-Qi, YIN Wu, WANG Kun, WANG Han, HU Bing
. Analysis of adult zebrafish OKR behavior[J]. Hereditas(Beijing), 2012
, 34(9)
: 1193
-1201
.
DOI: 10.3724/SP.J.1005.2012.01193
[1] Portugues R, Engert F. The neural basis of visual behaviors in the larval zebrafish. Curr Opin Neurobiol, 2009, 19(6): 644-647.
[2] Maurer CM, Huang YY, Neuhauss SC. Application of zebrafish oculomotor behavior to model human disorders. Rev Neurosci, 2011, 22(1): 5-16.
[3] Huang YY, Neuhauss SCF. The optokinetic response in zebrafish and its applications. Frontiers in Biosci-ence, 2008, 13: 1899-1916.
[4] Easter SS Jr, Nicola GN. The development of eye movements in the zebrafish (Danio rerio). Dev Psychobiol, 1997, 31(4): 267-276.
[5] Emran F, Rihel J, Adolph AR, Wong KY, Kraves S, Dowling JE. OFF ganglion cells cannot drive the optokinetic reflex in zebrafish. Proc Natl Acad Sci USA, 2007, 104(48): 19126-19131.
[6] Roeser T, Baier H. Visuomotor behaviors in larval zebrafish after GFP-guided laser ablation of the optic tectum. J Neurosci, 2003, 23(9): 3726-3734.
[7] Matsui JI, Egana AL, Sponholtz TR, Adolph AR, Dowling JE. Effects of ethanol on photoreceptors and visual function in developing zebrafish. Invest Ophthalmol Vis Sci, 2006, 47(10): 4589-4597.
[8] Neuhauss SCF. Behavioral genetic approaches to visual system development and function in zebrafish. J Neurobiol, 2003, 54(1): 148-160.
[9] Mueller KP, Neuhauss SC. Quantitative measurements of the optokinetic response in adult fish. J Neurosci Methods, 2010, 186(1): 29-34.
[10] Zou SQ, Yin W, Zhang MJ, Hu CR, Huang YB, Hu B. Using the optokinetic response to study visual function of zebrafish. J Vis Exp, 2010, doi: 10.3791/1742.
[11] Beck JC, Gilland E, Tank DW, Baker R. Quantifying the ontogeny of optokinetic and vestibuloocular behaviors in zebrafish, medaka, and goldfish. J Neurophysiol, 2004, 92(6): 3546-3561.
[12] Brockerhoff SE. Measuring the optokinetic response of zebrafish larvae. Nat Protoc, 2006, 1(5): 2448-2451.
[13] Rinner O, Rick JM, Neuhauss SCF. Contrast sensitivity, spatial and temporal tuning of the larval zebrafish optokinetic response. Invest Ophth Vis Sci, 2005, 46(1): 137-142.
[14] Emran F, Rihel J, Adolph AR, Dowling JE. Zebrafish larvae lose vision at night. Proc Natl Acad Sci USA, 2010, 107(13): 6034-6039.
[15] Easter SS, Nicola GN. The development of vision in the zebrafish (Danio rerio). Dev Biol, 1996, 180(2): 646-663.
[16] Cahill H, Nathans J. The optokinetic reflex as a tool for quantitative analyses of nervous system function in mice: application to genetic and drug-induced variation. PLoS One, 2008, 3(4): e2055.
[17] Qian H, Zhu Y, Ramsey DJ, Chappell RL, Dowling JE, Ripps H. Directional asymmetries in the optokinetic response of larval zebrafish (Danio rerio). Zebrafish, 2005, 2(3): 189-196.
[18] Thirumalai V, Cline HT. A commanding control of behavior. Nat Neurosci, 2008, 11(3): 246-248.
[19] Wang H. Comparative analysis of period genes in teleost fish genomes. J Mol Evol, 2008, 67(1): 29-40.
[20] DeBruyne J, Hurd MW, Gutierrez L, Kaneko M, Tan Y, Wells DE, Cahill GM. Isolation and phenogenetics of a novel circadian rhythm mutant in zebrafish. J Neurogenet, 2004, 18(2): 403-428.
[21] Vallone D, Gondi SB, Whitmore D, Foulkes NS. E-box function in a period gene repressed by light. Proc Natl Acad Sci USA, 2004, 101(12): 4106-4111.
