The genetic diversity of swine leukocyte antigen complex (SLA) was studied among Guangdong local pigs, Huanan wild boars (S.s. chirodontus) and introduced pigs, which aimed at providing a theoretical foundation for further pig anti-disease resistance breeding. Pietrain pigs, Duroc pigs, Large black-white pigs, Lantang pigs, and Huanan wild boars were genotyped by employing 18 microsatellites in swine leukocyte antigen complex (SLA-MS). The result showed that the average diversity in SLA II was higher (He=0.628, PIC=0.581) than that in SLA I (He=0.530, PIC=0.474) and in SLA III (He=0.526, PIC=0.458). The molecular diversity indices (MDI) of Huanan wild boars was the highest(0.716), followed by Lantang pigs (0.614), Large black-white pigs (0.559), Pietrain pigs (0.550) and Duroc pigs (0.507). As a whole, the genetic diversity of Huanan wild boars was the highest over Guangdong native pigs and introduced pigs. Large black-white pigs and Duroc pigs had ever happened a severe bottleneck by comparison with the Garza-Williamson index (GWI) in Huanan wild boar. From the genetic distance, one clade was that Lantang pigs were first clustered with Huanan wild boar, and then grouped together with Large black-white pigs; another clade was that Pietrain pigs were independently clustered with Duroc pigs in the NJ tree. The results would establish the foundation for pig conservation of germplasm resource, disease resistance breeding, and multiplicative strains.
[1] 于辉, 李彬, 李华, 颜其贵. SLA与免疫抗病的研究进展. 中国畜牧杂志, 2009, 45(5): 60-64.
[2] Lunney JK, Ho CS, Wysocki M, Smith DM. Molecular genetics of the swine major histocompatibility complex, the SLA complex. Dev Comp Immunol, 2009, 33(3): 362-374.
[3] Nuñez Y, Ponz F, Gallego FJ. Microsatellite-based genotyping of the swine lymphocyte alloantigens (SLA) in miniature pigs. Res Vet Sci, 2004, 77(1): 59-62.
[4] Tanaka M, Ando A, Renard C, Chardon P, Domukai M, Okumura N, Awata T, Uenishi H. Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds. Immunogenetics, 2005, 57(9): 690-696.
[5] Ando A, Uenishi H, Kawata H, Tanaka-Matsuda M, Shigenari A, Flori L, Chardon P, Lunney JK, Kulski JK, Inoko H. Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds. Immunogenetics, 2008, 60(7): 399-407.
[6] Ho CS, Martens GW, Amoss MS Jr, Gomez-Raya L, Beattie CW, Smith DM. Swine leukocyte antigen (SLA) diversity in Sinclair and Hanford swine. Dev Comp Immunol, 2010, 34(3): 250-257.
[7] Chung H, McClure MC. Characterization of microsatellite loci in the SLA class I region. Genomics, 2011, 97(4): 223-234.
[8] Gao Y, Wahlberg P, Marthey S, Esquerre D, Jaffrézic F, Lecardonnel J, Hugot K, Rogel-Gaillard C. Analysis of porcine MHC using microarrays. Vet Immunol Immunopathol, 2012, 148(1-2): 78-84.
[9] Lunney JK, Chen HB. Genetic control of host resistance to porcine reproductive and respiratory syndrome virus (PRRSV) infection. Virus Res, 2010, 154(1-2): 161-169.
[10] Excoffier L, Laval G, Schneider S. Arlequin ver. 3. 0: an integrated software package for population genetics data analysis. Evol Bioinform Online, 2005, 1: 47-50.
[11] Takezaki M, Nei M. Genetics distance and reconstruction of phylogenetic trees from microsatellite DNA. Genetics, 1996, 144(1): 389-399
[12] Nei M, Tajima F, Tateno Y. Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data. J Mol Evol, 1983, 19(2): 153-170.
[13] Frankham R, Ballou J, Briscoe D. Introduction to conservation genetics. Cambridge: Cambridge University Press, 2002
[14] 张桂香, 王志刚, 孙飞舟, 陈伟生, 杨国义, 郭式健, 李拥军, 赵小丽, 张沅, 孙军, 樊斌, 杨述林, 李奎. 56个中国地方猪种微卫星基因座的遗传多样性. 遗传学报, 2003, 30(3): 225-233.
[15] Fang M, Hu X, Jiang T, Braunschweig M, Hu L, Du Z, Feng J, Zhang Q, Wu C, Li N. The phylogeny of Chinese indigenous pig breeds inferred from microsatellite markers. Anim Genet, 2005, 36(1): 7-13.
[16] 郑丕留, 张仲葛, 陈效华, 涂友仁. 中国猪品种志. 上海: 上海科学技术出版社, 1986.
[17] 陈善元, 张亚平. 家养动物起源研究的遗传学方法及其应用. 科学通报, 2006, 51(21): 2469-2475.
[18] 常青, 周开亚. 分子进化研究中系统发生树的重建. 生物多样性, 1998, 6(1): 55-62.
[19] 姚绍宽, 张勤, 孙飞舟, 刘培琼. 利用微卫星标记分析7品种(类群)小型猪的遗传多样性. 遗传, 2006, 28(4): 407-412.
[20] SanCristobal M, Chevalet C, Peleman J, Heuven H, Brugmans B, van Schriek M, Joosten R, Rattink AP, Harlizius B, Groenen MAM, Amigues Y, Boscher MY, Russell G, Law A, Davoli R, Russo V, Desautes C, Alderson L, Fimland E, Bagga M, Delgado JV, Vega-Pla JL, Martinez AM, Ramos M, Glodek P, Meyer JN, Gandini G, Matassino D, Siggens K, Laval G, Archibald A, Milan D, Hammond K, Cardellino R, Haley C, Plastow G. Genetic diversity in European pigs utilizing amplified fragment length polymorphism markers. Anim Genet, 2006, 37(3): 232-238.
