[an error occurred while processing this directive]
en

Screening and identification of 36 new STR loci in human Y chro-mosome

Expand
  • 1. State Key Laboratory of Genetic Engineering, MOE Key Laboratory of Contemporary Anthropology, School of Life Science, Fudan University, Shanghai 200433, China 2. Center for Genetic Genomic Analysi, Genesky Biotechnologies Inc., Shanghai 201203, China

Received date: 2012-05-14

  Revised date: 2012-07-13

  Online published: 2012-11-25

Abstract

133 candidate Y-STR loci were selected from NCBI STS database or by bioinformatics analysis in human Y-chromosome sequence, and were screened among 48 DNA samples around the world. Forty-one Y-STRs with high allelic frequency were validated, 36 of which were first reported. Two hundred haplotypes of the 41 STRs were identified among 200 randomly sampled male individuals in Shanghai, indicating 100% inter-individual discrimination. By network analysis of haplotypes of the 41 STRs among nine Jiang-surname male individuals with no consanguinity within 5 generations from a Jiang-surname individual gathering at Jiangshan, Zhejiang Province, and 7 Jiang-surname male individuals from the random shanghai population, 6 Jiang-surname individuals from Jiangshan were close with only 2-4 STR locus difference. These 41 Y-STR loci provide enough information by which individuals from each other with different early modern family origin can be effectively distinguished. This will promote studies on identification of non-lineal relationship in forensics, ancestry location of oversea Chinese, the surname origin and evolution, origin and migration of modern humans and many other studies of Contemporary Anthropology.

Cite this article

PENG Dong-Bo, JIANG Zheng-Wen, SUN Si-Ping, LI Cai-Hua, LU Da-Ru . Screening and identification of 36 new STR loci in human Y chro-mosome[J]. Hereditas(Beijing), 2012 , 34(11) : 1409 -1416 . DOI: 10.3724/SP.J.1005.2012.01409

References

[1] Painter TS. The Y-Chromosome in mammals. Science, 1921, 27: 503-504.
[2] Skaletsky H, Kuroda-Kawaguchi T, Minx PJ, Cordum HS, Hillier L, Brown LG, Repping S, Pyntikova T, Ali J, Bieri T, Chinwalla A, Delehaunty A, Delehaunty K, Du H, Fewell G, Fulton L, Fulton R, Graves T, Hou SF, Latrielle P, Leonard S, Mardis E, Maupin R, McPherson J, Miner T, Nash W, Nguyen C, Ozersky P, Pepin K, Rock S, Rohlfing T, Scott K, Schultz B, Strong C, Tin-Wollam A, Yang SP, Waterston RH, Wilson RK, Rozen S, Page DC. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature, 2003, 423(6942): 825-837.
[3] Garrigan D, Hammer MF. Reconstructing human origins in the genomic era. Nat Rev Genet, 2006, 7(9): 669-680.
[4] Cavalli-Sforza LL. The human genome diversity project: past, present and future. Nat Rev Genet, 2005, 6(4): 333-340.
[5] Kayser M, Kittler R, Erler A, Hedman M, Lee AC, Mo-hyuddin A, Mehdi SQ, Rosser Z, Stoneking M, Jobling MA, Sajantila A, Tyler Smith C. A comprehensive survey of human Y-chromosomal microsatellites. Am J Hum Genet, 2004, 74(6): 1183-1197.
[6] 石美森, 百茹峰, 于晓军, 唐剑频. 广东汉族22个Y-STR基因座遗传多态性及遗传关系分析. 遗传, 2008, 30 (9): 1136-1142.
[7] 石美森, 百茹峰, 张金生, 于晓军. 辽宁满族11个Y-STR基因座多态性及遗传关系的分析. 遗传, 2008, 30(5): 583-589.
[8] 冯冬亮, 刘长晖, 梁祚仁, 刘超. 广西4个少数民族17个Y-STR基因座的多态性分析. 遗传, 2009, 31(9): 921-935.
[9] 黄艳梅, 祁英杰, 朱运良, 童大跃, 伍新尧. 7个Y-STR基因座荧光标记复合分型及在中国汉族群体中遗传多态性的研究. 遗传, 2007, 29(10): 1214-1222.
[10] 何 燕, 单可人, 谢渊, 修瑾, 吴昌学, 李毅, 任锡麟. 贵州三都水族Y染色体上七个STR基因座的遗传多态性分析. 遗传, 2006, 28(12): 1495-1499.
[11] 黄艳梅, 祁英杰, 徐基清, 朱运良, 伍新尧. 2个新Y-STR基因座的序列分析和在广东汉族群体中的多态性研究. 遗传, 2006, 28(11): 1355-1360.
[12] Grskovi? B, Mrsi? G. Y chromosome: from evolution to forensics- an overview. Acta Med Croatica, 2010, 64(1): 33-40.
[13] McElreavey K, Quintana-Murci L. A population genetics perspective of the Indus valley through uniparentally-inherited markers. Ann Hum Biol, 2005, 32(2): 154-162.
[14] Gemmell NJ, Sin FY. Mitochondrial mutations may drive Y chromosome evolution. Bioessays, 2002, 24(3): 275-279.
[15] Kayser M, Roewer L, Hedman M, Henke L, Henke J, Brauer S, Krüger C, Krawczak M, Nagy M, Dobosz T, Szibor R, de Knijff P, Stoneking M, Sajantila A. Charaeteristics and frequency of germline mutations at microsatellite loci from the human Y chromosome, as revealed by direct observation in father/son Pairs. Am J Hum Genet, 2000, 66(5): 1580-1588.
[16] 石美森, 百茹峰, 傅博. 山西汉族17个Y-STR基因座遗传多态性及遗传关系. 遗传, 2011, 33(3): 228-238.
[17] Bär W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B, DNA recommendations. Further report of the DNA Commission of the ISFG re-garding the use of short tandem repeat systems. Fo-rensic Sci Int, 1997, 87(3): 179-184.
[18] Perici? M, Bara? Lauc L, Martinovi? Klari? I, Jani?ijevi? B, Rudan P. Review of Croatian genetic heritage as revealed by mitochondrial DNA and Y chromosomal line-ages. Croat Med J, 2005, 46(4): 502-513.
[19] Scheinfeldt L, Friedlaender F, Friedlaender J, Latham K, Koki G, Karafet T, Hammer M, Lorenz J. Unexpected NRY chromosome variation in Northern Island melanesia. Mol Biol Evol, 2006, 23(8): 1628-1641.
[20] Bandelt HJ, Forster P, Röhl A. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol, 1999, 16(1): 37-48.
Outlines

/