The grasshoppers are ideal materials to study various meiotic stages of spermatogenesis due to their easy availability, fairly large chromosomes, and fewer numbers of chromosomes. It is easy to make temporary squash preparation of grasshopper testes; however, it is usually difficult for the beginners to differentiate between stages of meiosis. In view of this, we demonstrated the method of identification of meiotic stages by chromosome number and chromosome conformation, taking spermatogonial meiosis of Locusta migratoria manilensis as an example. We described briefly the mitosis of spermatogonia and the spermatogenesis of this species as well.
LIU Meng-Hao ZHAO Kai-Qiang WANG Ya-Dong YANG Meng-Ping ZHAO Ning-Ning YANG Da-Xiang
. Identification of the meiotic events in grasshopper spermatogenesis[J]. Hereditas(Beijing), 2012
, 34(12)
: 1628
-1637
.
DOI: 10.3724/SP.J.1005.2012.01628
[1] Martin JM. The use of grasshopper chromosomes to demonstrate meiosis. Tuatara, 1970, 18(1): 1-12.
[2] 郭郛, 陈永林, 卢宝廉. 中国飞蝗生物学. 山东: 山东科学技术出版社, 1991.
[3] 赵卓, 任炳忠, 奚耕思. 绿牧草蝗精子发生观察. 吉林农业大学学报, 2005, 27(6): 603-605.
[4] Dyer AF. Investigating Chromosomes. London: Edward Arnold, 1979.
[5] John B. Meiosis. Cambridge: Cambridge University Press, 1990: 29-102.
[6] 龚玉新, 吴丹丹. 蝗虫的减数分裂观察. 生物学通报, 2008, 43(4): 56-58.
[7] 王佑举, 贾玉珩. 蝗虫减数分裂的观察. 生物学通报, 1985, (12): 41, 47.
[8] 姚世鸿. 云斑车蝗精母细胞减数分裂过程的观察与分析. 贵州师范大学学报(自然科学版), 1994, 12(1): 27-32.
[9] 陈宝瑄. 蝗虫生殖细胞减数分裂及精子形成标本的制作与辨认方法. 河北医学院学报, 1986, 7(1): 47-78.
[10] 杨大翔. 遗传学实验(第二版). 北京: 科学出版社, 2009: 80-90.
[11] 贾潇凌, 马恩波. 斑翅蝗科部分种属细胞分类学的研究. 山西大学学报(自然科学版), 1998, 21(3): 291-296.
[12] de Souza MJ, de Melo NF. Chromosome study in Schistocerca (Orthop-tera-Acrididae-Cyrtacanthacridinae): Karyotypes and distri-bution patterns of constitutive heterochromatin and nucleolus organizer regions (NORs). Genet Mol Biol, 2007, 30(1): 54-59.
[13] 马恩波, 姚爱玉. 蝗虫染色体分带技术的研究. 遗传, 1999, 21(3): 28-30.
[14] Perry PE, Jones GH. Male and female meiosis in grass-hoppers. I. Stethophyma grossum. Chromosoma, 1974, 47(3): 227-236.
[15] 葛少钦, 康现江, 刘桂荣, 穆淑梅. 精子发生过程中的相关基因. 遗传, 2008, 30(1): 3-12.
[16] 葛少钦, 李建忠, 张晓静. 精子发生过程中组蛋白甲基化和乙酰化. 遗传, 2011, 33(9): 939-946.