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A new agroinoculation technology for foreign gene expression in plants by means of transient expression

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  • The School of Life Science, Jilin Normal University, Siping 136000, China

Received date: 2012-09-13

  Revised date: 2012-10-19

  Online published: 2013-01-25

Abstract

Due to the laborious and scale-up limitation we have developed a simple method named “seed absorption” to express foreign proteins in plants by means of transient expression. It has been shown that the reporter gene GFP was ex-pressed successfully in tomato (Solanum lycopersicum L.) plants by seed absorbing Agrobacterium suspension containing TMV-based p35S-30B-GFP vector. Various factors influencing the gene expression were optimized including Agrobacterium cell density and other inoculation conditions. This method has the special advantages as simple work process, ease to scale-up, and further expanding the host range of plant bioreactor than previous methods. We assume that the seed absorption method will facilitate the industrial scale production of the recombinant pharmaceutical proteins in plants.

Cite this article

YANG Li-Ping JIN Tai-Cheng XU Hong-Wei LI Hua ZHOU Xiao-Fu . A new agroinoculation technology for foreign gene expression in plants by means of transient expression[J]. Hereditas(Beijing), 2013 , 35(1) : 111 -117 . DOI: 10.3724/SP.J.1005.2013.00111

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