Isolation and biological characterization of mesenchymal stem cells from Beijing fatty chicken fetal liver

MU Ren BIAN Yan-Chao PU Ya-Bin LI Xiang-Chen WANG Feng-Long GUAN Wei-Jun

doi:10.3724/SP.J.1005.2013.00365

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Hereditas(Beijing) >

2013 , Vol. 35 >Issue 3: 365 - 372

DOI: https://doi.org/10.3724/SP.J.1005.2013.00365

Isolation and biological characterization of mesenchymal stem cells from Beijing fatty chicken fetal liver

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1. College of Animal Veterinary, Inner Mongolia Agricultural University, Hohhot 010018, China 2. Institute of Beijing Animal Science and Veterinary, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2012-08-31

Revised date: 2012-10-10

Online published: 2013-03-25

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Abstract

Mesenchymal stem cells (MSCS) from chicken fetal liver are multipotent stem cells that can differentiate in vitro into various terminally differentiated cells. The majority of studies have focused on rats and mice now. Reports from other animals are less and analyses on domestic animals are few. In this study, chicken liver-derived MSCs were isolated from 7-day-old embryo of Beijing fatty chickens. Primary liver-derived MSCs were subcultured to passage 15. The surface markers of liver-derived MSCs, CD29, and CD44 were detected by immunofluorescence and the surface markers CD34 and CK19 of hematopoietic progenitor cells/hepatic oval cells were not detected. RT-PCR analysis detected positive expression of CD29, CD44, CD71, and CD73. The growth curves were typically sigmoidal. Liver-derived MSCs of different passages were successfully induced and differentiated into neuronal and osteoblast cells. The results suggest that the MSCs isolated from chicken fetal liver possess similar biological characteristics with those derived from mice, and their multilineage dif-ferentiation provides many potential applications.

Key words： Beijing fatty chicken; mesenchymal stem cells; proliferation; differentiation potential

Cite this article

MU Ren BIAN Yan-Chao PU Ya-Bin LI Xiang-Chen WANG Feng-Long GUAN Wei-Jun . Isolation and biological characterization of mesenchymal stem cells from Beijing fatty chicken fetal liver[J]. Hereditas(Beijing), 2013 , 35(3) : 365 -372 . DOI: 10.3724/SP.J.1005.2013.00365

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