Whole mount in situ hybridization (WISH) is a popular technique that is used to study temporal-spatial gene expression at mRNA level in model systems. Sensitive and high-resolution WISH is important for every laboratory that uses this technique to study expression pattern of genes in wildtype and perturbed embryos. Based on the commonly used proto-cols in fish community, our lab optimizes them to get more sensitive and specific results. Here, we briefly summarize the history of this technology using zebrafish as an example, describe a detailed experimental protocol, and provide trouble-shooting for imperfect results and their solution.
ZHANG Chun-Xia, LIU Feng
. A brief protocol for high-resolution whole mount in situ hybridization in zebrafish[J]. Hereditas(Beijing), 2013
, 35(4)
: 522
-528
.
DOI: 10.3724/SP.J.1005.2013.00522
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