Correlation analysis between polymorphism of the 59383635th locus on X chromosome and fat-tail trait in sheep
Received date: 2013-07-01
Revised date: 2013-08-06
Online published: 2013-10-25
Fat tail or fat rump is one of essential traits for surviving in harsh environments, and the mechanism of fat deposition and its inheritable characters in sheep are still unclear. Therefore, the 59383635th locus on X chromosome in our unpublished chip data was chosen as candidate SNP, PCR-SSCP method was used to detect genotypes in five sheep breeds which have extreme differences in tail types (Altay, Small Tail Han Sheep, Hu, Chinese Merino and Suffolk), and the mathematical model was employed to analyze the correlation between the polymorphism and the trait of fat tail or fat rump. The results in this study showed that the high frequency of allele T exists in Altay flock, and the frequency of allele C appears to be particularly high in the thin tail sheep breeds. The result of mathematical model showed that the ratio of T/C increased exponentially with the increase of phenotype score. These results suggest that there is a big difference in the SNP distribution between fat tail (rump) and thin tail sheep populations, and the SNP can be used as an ideal molecular marker in high-fat or low-fat sheep breeding. However, the biological function of the SNP remains to be further studied.
Key words： fat tail trait; Altay sheep; polymorphism; genotype
GAN Shang-Quan ZHANG Wei SHEN Min LI Huan YANG Jing-Quan LIANG Yao-Wei GAO Lei LIU Shou-Ren WANG Xin-Hua . Correlation analysis between polymorphism of the 59383635th locus on X chromosome and fat-tail trait in sheep[J]. Hereditas(Beijing), 2013 , 35(10) : 1209 -1216 . DOI: 10.3724/SP.J.1005.2013.01209
[1] Chessa B, Pereira F, Arnaud F, Amorim A, Goyache F, Mainland I, Kao RR, Pemberton JM, Beraldi D, Stear MJ, Alberti A, Pittau M, Iannuzzi L, Banabazi MH, Kazwala RR, Zhang YP, Arranz JJ, Ali BA, Wang ZL, Uzun M, Dione MM, Olsaker I, Holm LE, Saarma U, Ahmad S, Marzanov N, Eythorsdottir E, Holland MJ, Ajmone-Marsan P, Bruford MW, Kantanen J, Spencer TE, Palmarini M. Re-vealing the history of sheep domestication using retrovirus integrations. Science, 2009, 324(5926): 532–536.<\p>
[2] Lawson Handley LJ, Byrne K, Santucci F, Townsend S, Taylor M, Bruford MW, Hewitt GM. Genetic structure of European sheep breeds. Heredity, 2007, 99(6): 620–631.<\p>
[3] Ryder ML. Sheep and Man. London: Duckworth Press, 1983.<\p>
[4] Dacidson A. The Oxford Companion to Food. 2nd ed. London: Oxford University Press, 2006.<\p>
[5] 国家畜禽遗传资源委员会. 中国畜禽遗传资源志. 北京: 中国农业出版社, 2011.<\p>
[6] 夏凯凯, 张佳兰. 阿勒泰羊品种生产现状及发展趋势. 科技信息, 2012, (34): 485–486.<\p>
[7] 王大星, 徐冬. 阿勒泰羊品种遗传资源调查报告. 草食家畜, 2009, (2): 38–40.<\p>
[8] Gökdal O, Atay O, Ülker H, Kayaardi S, Kanter M, DeAvila MD, Reeves JJ. The effects of immunological castration against GnRH with recombinant OL protein (Ovalbumin-LHRH-7) on carcass and meat quality char-acteristics, histological appearance of testes and pituitary gland in Kivircik male lambs. Meat Sci, 2010, 86(3): 692–698.<\p>
[9] 许莲萍, 王文均, 郭晓昭, 姚刚. 断奶对阿勒泰羔羊血液生理生化指标的影响. 新疆农业大学学报, 2007, 30(2): 71–73.<\p>
[10] 魏彬, 刘志强, 贝念湘, 李新平, 黄俊成, 叶林. 阿勒泰羊生理生化血液流变学常值. 草食家畜, 1997, (1): 41–44.<\p>
[11] Hu ZL, Park CA, Wu XL, Reecy JM. Animal QTLdb: an improved database tool for livestock animal QTL/association data dissemination in the post-genome era. Nucl Acids Res, 2013, 41(D1): D871–D879.<\p>
[12] Rohrer GA, Keele JW. Identification of quantitative trait loci affecting carcass composition in swine: I. Fat deposi-tion traits. J Anim Sci, 1998, 76(9): 2247–2254.<\p>
[13] Rohrer GA, Keele JW. Identification of quantitative trait loci affecting carcass composition in swine: II. Muscling and wholesale product yield traits. J Anim Sci, 1998, 76(9): 2255–2262.<\p>
[14] Moradi MH, Nejati-Javaremi A, Moradi-Shahrbabak M, Dodds KG, McEwan JC. Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition. BMC Genet, 2012, 13(1): 10, doi: 10.1186/1471-2156-13-10.<\p>
[15] Raoofi A, Shahraki AR, Namjoo A, Momtaz H. A survey on prevalence and pathological findings of gallstones in Lori-Bakhtiari sheep in Iran. The Scientific World J, 2012, doi: 10.1100/2012/524607.<\p>
[16] Farhangfar H, Naeemipour H, Zinvand B. Application of random regression model to estimate genetic parameters for average daily gains in Lori-Bakhtiari sheep breed of Iran. Pakistan J Biol Sci, 2007, 10(14): 2407–2412.<\p>
[17] 赵倩君, 关伟军, 乔海云, 孟详人, 韩建林, 李向臣, 何晓红, 浦亚斌, 马月辉. 基于cytb基因探讨家绵羊和多角绵羊的系统发育. 中国农业科学, 2010, 43(14): 3005–3011.<\p>
[18] 巩元芳, 李祥龙, 刘铮铸, 李金泉. 我国主要地方绵羊品种随机扩增多态DNA研究. 遗传, 2002, 24(4): 423– 426.<\p>
[19] Miles EL, O'Gorman C, Zhao JG, Samuel M, Walters E, Yi YJ, Sutovsky M, Prather RS, Wells KD, Sutovsky P. Transgenic pig carrying green fluorescent proteasomes. Proc Natl Acad Sci USA, 2013, 110(16): 6334–6339.<\p>
[20] Pihlajamäki J, Boes T, Kim EY, Dearie F, Kim BW, Schroeder J, Mun E, Nasser I, Park PJ, Bianco AC, Gold-fine AB, Patti ME. Thyroid hormone-related regulation of gene expression in human fatty liver. J Clin Endocrinol Metab, 2009, 94(9): 3521–3529.<\p>
[21] Beauchamp NJ, Daly ME, Makris M, Preston FE, Peake IR. A novel mutation in intron K of the PROS1 gene causes aberrant RNA splicing and is a common cause of protein S deficiency in
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