The application of MutMap in forward genetic studies based on whole-genome sequencing
Received date: 2017-03-18
Revised date: 2017-05-31
Online published: 2017-08-07
Supported by
the National Natural Science Foundation of China-Henan Talent Training Joint Foundation(U1504319);the National Natural Science Foundation of China(31670317);the Key Research Fund of the Higher Education Institutions of Henan Province(15A180044);the Program for Innovative Research Team (in Science and Technology) in University of Henan Province(17IRTSTHN017)
Classical forward genetic analysis relies on construction of complicated progeny populations and development of many molecular markers for linkage analysis in genetic mapping, which is both time- and cost-consuming. The recently developed MutMap is a new forward genetic approach based on high-throughput next-generation sequencing technologies. It is more efficient and affordable than traditional methods. Moreover, new extended methods based on MutMap have been developed: MutMap+, which is based on self-crossing; MutMap-Gap, which is used to recognize the causative variations occurring in genome gap regions; QTL-seq, a method similar to MutMap for mapping quantitative trait loci. These methods are free from constructing complicated mapping population, genetic hybridization and linkage information. They have greatly accelerated the identification of genetic elements associated with interested phenotypic variation. Here, we review the basic principles of MutMap, and discuss their future applications in next generation sequencing-based forward genetic mapping and crop improvement.
Key words： MutMap; mutant; quantitative trait locus; whole-genome resequencing
Jinhong Yuan, Junhua Li, Jiaojiao Yuan, Keli Jia, Shufen Li, Chuanliang Deng, Wujun Gao . The application of MutMap in forward genetic studies based on whole-genome sequencing[J]. Hereditas(Beijing), 2017 , 39(12) : 1168 -1177 . DOI: 10.16288/j.yczz.17-095
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