Bacillus anthracis, B. thuringiensis and B. cereus are members of the B. cereus group. They share high genetic similarity. Whereas plcR (Phospholipase C regulator) usually encodes a functional pleiotropic activator protein in B. cereus and B. thuringiensis isolates, a characteristic nonsense mutation is found in all B. anthracis strains investigated, making the gene dysfunctional. To study the function of PlcR in B. anthracis, we used the B. cereus CMCC63301 genome as a template and constructed a recombinant expression plasmid pBE2A-plcR, and introduced it into the B. anthracis vaccine strain A16R, and then analyzed the activity of the hemolysin and sphingomyelinase. The results showed that transformation of B. anthracis with plasmid pBE2A-plcR carrying the native B. cereus plcR gene active the expression of sphingomyelinase gene, but did not activate expression of hemolysin genes of B. anthracis A16R.
Xiaolin Jia, Dongshu Wang, Zhiqi Gao, Erling Feng, Jiping Zheng, Hengliang Wang, Guiying Guo, Xiankai Liu
. The function of PlcR in Bacillus anthracis vaccine strain A16R[J]. Hereditas(Beijing), 2015
, 37(5)
: 494
-498
.
DOI: 10.16288/j.yczz.15-040
[1] 王玲, 陈亚华, Mahillon J, 喻子牛. 蜡状芽胞杆菌转录激活子PIcR调控多基因的表达. 微生物学报, 2001, 41(3): 304-309.
[2] 黄必旺, 邵恩斯, 蔡瑞, 苏芙蓉, 关雄. 苏云金杆菌毒素调控基因 plcR 的特性与表达. 激光生物学报, 2010, 19(6): 798-803.
[3] Slamti L, Perchat S, Gominet M, Vilas-Bôas G, Fouet A, Mock M, Sanchis V, Chaufaux J, Gohar M, Lereclus D. Distinct mutations in PlcR explain why some strains of the Bacillus cereus group are nonhemolytic. J Bacteriol , 2004, 186(11): 3531-3538.
[4] Gohar M, Økstad OA, Gilois N, Sanchis V, Kolstø AB, Lereclus D. Two-dimensional electrophoresis analysis of the extracellular proteome of Bacillus cereus reveals the importance of the PlcR regulon. Proteomics , 2002, 2: 784-791.
[5] Mignot T, Mock M, Robichon D, Landier A, Lereclus D, Fouet A. The incompatibility between the PlcR- and AtxA-controlled regulons may have selected a nonsense mutation in Bacillus anthracis . Mol Microbiol , 2001, 42(5): 1189-1198.
[6] 郭兴华, 熊占, 周民, 贾士芳, 许怡. 枯草杆菌-大肠杆菌多功能穿梭载体的构建. 生物工程学报, 1991, 7(3): 224-229.
[7] Grenha R, Slamti L, Nicaise M, Refes Y, Lereclus D, Nessler S. Structural basis for the activation mechanism of the PlcR virulence regulator by the quorum-sensing signal peptide PapR. Proc Natl Acad Sci USA , 2013, 110(3): 1047-1052.
[8] Agaisse H, Gominet M, Økstad OA, Kolstø AB, Lereclus D. PlcR is a pleiotropic regulator of extracellular virulence factor gene expression in Bacillus thuringiensis . Mol Microbiol , 1999, 32(5): 1043-1053.
[9] Pomerantsev AP, Pomerantseva OM, Leppla SH. A spontaneous translational fusion of Bacillus cereus PlcR and PapR activates transcription of PlcR-dependent genes in Bacillus anthracis via binding with a specific palindromic sequence. Infect Immun , 2004, 72(10): 5814-5823.
[10] Slamti L, Lereclus D. A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. EMBO J , 2002, 21(17): 4550-4559.
[11] Sastalla I, Maltese LM, Pomerantseva OM, Pomerantsev AP, Keane-Myers A, Leppla SH. Activation of the latent PlcR regulon in Bacillus anthracis . Microbiology , 2010, 156(10): 2982-2993.
[12] Ko KS, Kim JW, Kim JM, Kim W, Chung SI, Kim IJ, Kook YH. Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR gene. Infect Immun , 2004, 72(9): 5253-5261.
