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Research Articles

Genetic analysis and identification of candidate genes for a narrow leaf mutant (zy17) in rice

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  • 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources and Agricultural College of Hainan University, Haikou 570228, China;
    2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Received date: 2015-02-02

  Revised date: 2015-04-03

  Online published: 2015-04-28

Abstract

Control of organ size by cell proliferation and cell expansion is a fundamental process in plant development, but little is known about the genetic and molecular mechanisms that determine organ size in plants. To understand the genetic and molecular mechanisms of organ growth control, we isolate a set of mutants with altered leaf size and identify the narrow leaf mutant, zhaiye 17 (zy17) (zhaiye means narrow leaf in Chinese). zy17 exhibits narrow leaves, slightly short plants, small panicles, reduced panicle branches and decreased grain numbers per panicle compared with the wild type. Our cytological analyses show that the narrow leaf phenotype of zy17 is caused by the reduced number of cells, indicating that ZY17 regulates cell proliferation. Genetic analyses show that the zy17 mutant phenotypes are controlled by a single gene. Using the whole genome resequencing approach and linkage analysis, we identify Os02g22390, Os02g28280 and Os02g29530 as candidate genes. Os02g22390 encodes a retrotransposon protein with the mutation occurring in the intronic region; Os02g28280 encodes a protein with unknown function with a base substitution resulting in non-synonymous mutation; Os02g29530 encodes a protein containing the PFAM domain related to glycosyltransferase, with a 2 bp deletion mutation causing a premature termination. Further studies on these three candidate genes will be helpful for understanding the molecular mechanism of organ size control in rice.

Cite this article

Xueqian Li, Ran Xu, Penggen Duan, Yingbao Wu, Yuehua Luo, Yunhai Li . Genetic analysis and identification of candidate genes for a narrow leaf mutant (zy17) in rice[J]. Hereditas(Beijing), 2015 , 37(6) : 582 -589 . DOI: 10.16288/j.yczz.15-056

References

[1] Tester M, Langridge P. Breeding technologies to increase crop production in a changing world. Science , 2010, 327(5967): 818-822.
[2] Foley JA, Ramankutty N, Brauman KA, Cassidy ES, Gerber JS, Johnston M, Mueller ND, O'Connell C, Ray DK, West PC, Balzer C, Bennett EM, Carpenter SR, Hill J, Monfreda C, Polasky S, Rockström J, Sheehan J, Siebert S, Tilman D, Zaks DP. Solutions for a cultivated planet . Nature , 2011, 478(7369): 337-342.
[3] 朱雄涛, 汪真. 水稻高光效生理育种初探 . 福建稻麦科技, 2003, 21(2): 14-17.
[4] 陈代波, 程式华, 曹立勇. 水稻窄叶性状的研究进展. 中国稻米, 2010, 16(3): 1-4.
[5] 王兰, 黄李超, 代丽萍, 杨窑龙, 徐杰, 冷语佳, 张光恒, 胡江, 朱丽, 高振宇, 董国军, 郭龙彪, 钱前, 曾大力. 利用日本晴/9311重组自交系群体定位水稻成熟期叶形相关性状QTL. 中国水稻科学, 2014, 28(6): 589-597.
[6] Chen ML, Luo J, Shao GN, Wei XJ, Tang SQ, Sheng ZH, Song J, Hu PS. Fine mapping of a major QTL for flag leaf width in rice, qFLW4 , which might be caused by alternative splicing of NAL1. Plant Cell Rep , 2012, 31(5): 863-872.
[7] Qi J, Qian Q, Bu QY, Li SY, Chen Q, Sun JQ, Liang WX, Zhou YH, Chu CC, Li XG, Ren FG, Palme K, Zhao BR, Chen JF, Chen MS, Li CY. Mutation of the rice Narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport . Plant Physiol , 2008, 147(4): 1947-1959.
[8] Woo YM, Park HJ, Su'udi M, Yang JI, Park JJ, Back K, Park YM, An G. Constitutively wilted 1, a member of the rice YUCCA gene family, is required for maintaining water homeostasis and an appropriate root to shoot ratio. Plant Mol Biol , 2007, 65(1-2): 125-136.
[9] Fujino K, Matsuda Y, Ozawa K, Nishimura T, Koshiba T, Fraaije MW, Sekiguchi H. NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics , 2008, 279(5): 499-507.
[10] Zhang GH, Li SY, Wang L, Ye WJ, Zeng DL, Rao YC, Peng YL, Hu J, Yang YL, Xu J, Ren DY, Gao ZY, Zhu L, Dong GJ, Hu XM, Yan MX, Guo LB, Li CY, Qian Q. LSCHL4 from Japonica cultivar, which is allelic to NAL1 , increases yield of indica super rice 93-11. Mol Plant , 2014, 7(8): 1350-1364.
[11] Fujita D, Trijatmiko KR, Tagle AG, Sapasap MV, Koide Y, Sasaki K, Tsakirpaloglou N, Gannaban RB, Nishimura T, Yanagihara S, Fukuta Y, Koshiba T, Slamet-Loedin IH, Ishimaru T, Kobayashi N. NAL1 allele from a rice landrace greatly increases yield in modern indica cultivars. Proc Natl Acad Sci USA , 2013, 110(51): 20431-20436.
[12] Li W, Wu C, Hu GC, Xing L, Qian WJ, Si H, Sun ZX, Wang XC, Fu YP, Liu WZ. Characterization and fine mapping of a novel rice narrow leaf mutant nal9. J Integr Plant Biol , 2013, 55(11): 1016-1025.
[13] Cho SH, Yoo SC, Zhang HT, Pandeya D, Koh HJ, Hwang JY, Kim GT, Paek NC. The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol , 2013, 198(4): 1071-1084.
[14] Hu J, Zhu L, Zeng DL, Gao ZY, Guo LB, Fang YX, Zhang GH, Dong GJ, Yan MX, Liu J, Qian Q. Identification and characterization of NARROW AND ROLLED LEAF 1 , a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol , 2010, 73(3): 283-292.
[15] Wu C, Fu YP, Hu GC, Si H, Cheng SH, Liu WZ. Isolation and characterization of a rice mutant with narrow and rolled leaves . Planta , 2010, 232(2): 313-324.
[16] Li M, Xiong GY, Li R, Cui JJ, Tang D, Zhang BC, Pauly M, Cheng ZK, Zhou YH. Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth. Plant J , 2009, 60(6): 1055-1069.
[17] Ding ZQ, Lin ZF, Li Q, Wu H, Xiang CY, Wang JF. DNL1, encodes cellulose synthase-like D4, is a major QTL for plant height and leaf width in rice ( Oryza sativa L.). Biochem Biophys Res Commun , 2015, 457(2): 133-140.
[18] Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M, Kitano H, Matsuoka M. A rice tryptophan deficient dwarf mutant, tdd1 , contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. Plant J ,
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